Ubiquitin C-terminal Hydrolase 1 (PGP9.5) Antibody, Mouse

Catalog #: 2060-UCHL1 Categories: , , , Datasheet:

$109.00$365.00

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Great marker for neuronal degeneration!

 

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:5000IHC 1:500-1:5000 ICC 1:500-1:2000
Species:
Mouse monoclonal
Gene Name:
UCHL1
Molecular Weight:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2060-UCHL1, RRID:AB_2492280
Antigen/Purification: ExpandCollapse

The antigen is a  recombinant full length human UCHL1 purified from E. coli.

The antibody is protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson’s disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.

Synonyms: ExpandCollapse

• Epididymis luminal protein 117 antibody
• Epididymis secretory protein Li 53 antibody
• HEL 117 antibody
• HEL S 53 antibody
• NDGOA antibody
• Neuron cytoplasmic protein 9.5 antibody
• OTTHUMP00000218137 antibody
• OTTHUMP00000218139 antibody
• OTTHUMP00000218140 antibody
• OTTHUMP00000218141 antibody
• Park 5 antibody
• PARK5 antibody
• PGP 9.5 antibody
• PGP9.5 antibody
• PGP95 antibody
• Protein gene product 9.5 antibody
• Ubiquitin C terminal esterase L1 antibody
• Ubiquitin C terminal hydrolase antibody
• Ubiquitin C terminal hydrolase L1 antibody
• Ubiquitin carboxyl terminal esterase L1 antibody
• Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
• Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
• Ubiquitin thioesterase L1 antibody
• Ubiquitin thiolesterase antibody
• Ubiquitin thiolesterase L1 antibody
• UCH-L1 antibody
• UCHL1 antibody
• UCHL1_HUMAN antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HEK 293 cells

Immunohistochemistry

Rat hippocampus (free floating section)
Rat spinal cord

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983). PMID: 6343558

Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuronspecific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989). PMID: 2530630

Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 10:1963-70 (2001). PMID: 11555633

Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson’s disease susceptibility. Cell 111:209-18 (2002). PMID: 12408865

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Ubiquitin C-terminal Hydrolase 1 (PGP9.5) Antibody, Mouse
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunolabeling of HEK 293 cells labeled with anti-UCHL1 antibody (catalog #2060-UCHL1, green, 1:500) and anti-neuron specific enolase antibody (cat #1520-NSE, red, 1:500). The blue is staining nuclear DNA.

Download the protocol used for this image:

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