Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase (Ser40) Antibody

We are the original manufacturer of our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific antibody. It is affinity purified from pooled serum, and great in WB, IHC, and ICC.

Catalog #: p1580-40 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rabbit, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:1000IHC 1:1000ICC 1:50-1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
TH
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1580-40, RRID:AB_2492279
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat PC-12 Cells.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Western Blot: Human | Mouse | Rat


Immunocytochemistry: Human
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.


Immunocytochemistry: Rat
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
10936746 1:300 Witkovsky, P, et al. 2000 Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of Chemical Neuroanatomy, 19(2), pp.105-116.


Western Blot: Human
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of Neurochemistry, 79(2), pp.349-360.


Western Blot: Mouse
PMID Dilution Publication
30803445 1:1000 Areal, L.B., et al. 2019 Neuronal scaffolding protein spinophilin is integral for cocaine-induced behavioral sensitization and ERK1/2 activation. Molecular Brain, 12(1), p.15.
29782882 1:1000 Salvatore, M.F., et al. 2018 Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128.
29299922 not listed Bezem, M.T., et al. 2018 Stabilization of human tyrosine hydroxylase in maltodextrin nanoparticles for delivery to neuronal cells and tissue. Bioconjugate Chemistry Feb 21;29(2):493-502.
27124386 not listed Salvatore, M.F., et al. 2016 Regulation of tyrosine hydroxylase expression and phosphorylation in dopamine transporter-deficient mice. ACS Chemical Neuroscience, 7(7), pp.941-951.
19017804 not listed Meyer, D.A., et al. 2008 Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 – 18566.


Western Blot: Rat
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
28637176 1:1000 Salvatore, M.F., et al. 2017 Dissociation of striatal dopamine and tyrosine hydroxylase expression from aging-related motor decline: evidence from calorie restriction intervention. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, Dec 12;73(1):11-20.
26777664 1:1000 Baroso-Chinea, P., et al. 2016 Long-term controlled GDNF over-expression reduces dopamine transporter activity without affecting tyrosine hydroxylase expression in the mesostriatal system rat. Neurobiology of Disease, 88:44-54.
24743698 not listed Izumi, Y, et al. 2014 Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478.
24410633 1:1000 Salvatore, M.F., et al. 2014 ser31 tyrosine hydroxylase phosphorylation parallels differences in dopamine recovery in nigrostriatal pathway following 6‐OHDA lesion. Journal of Neurochemistry, 129(3), 548-558.
22723669 1:100,000 McCutcheon, J.E., et al. 2012 Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults. Journal of Neurophysiology 108(6): 1620-1630.
22458761 1:2000 Calipari, E.S., et al. 2014 Methylphenidate and cocaine self‐administration produce distinct dopamine terminal alterations. Addiction Biology, 19(2), pp.145-155.
12124439 1:1500 Jedynak, J.P., et al. 2002 Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of Neurochemistry, 82(2), pp.382-388.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of Neurochemistry, 79(2), pp.349-360.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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