Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase (Ser31) Antibody

Catalog #: p1580-31 Category: Datasheet:

$109.00$365.00

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Phospho-specific TH antibody!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Species Reactivity:
Non-human primate
Applications:
WB 1:1000IHC 1:300-1:1000ICC 1:50-1:300 Don't see your application?
Species:
Rabbit
Gene Name:
TH
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1580-31, RRID:AB_2492278
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat tyrosine hydroxylase (TH).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat PC12 Cells.

Immunohistochemistry

Rat retina

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

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Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Rat
Western Blot: Human | Mouse | Rat


Immunocytochemistry: Human
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.


Immunocytochemistry: Rat
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
10936746 1:300 Witkovsky, P, et al. 2000 Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116.


Immunohistochemistry: Rat
PMID Dilution Publication
10936746 1:300 Witkovsky, P, et al. 2000 Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116.


Western Blot: Human
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.


Western Blot: Mouse
PMID Dilution Publication
29782882 1:1000 Salvatore, M.F., et al. 2018 Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128.


Western Blot: Rat
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
24743698 not listed Izumi, Y, et al. 2014 Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478.
22458761 1:2000 Calipari, E.S., et al. 2014 Methylphenidate and cocaine self‐administration produce distinct dopamine terminal alterations. Addiction biology, 19(2), pp.145-155.
12124439 1:1500 Jedynak, J.P., et al. 2002 Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of neurochemistry, 82(2), pp.382-388.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tyrosine Hydroxylase ser31 Antibody
Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31.

Click here to view our Western blotting and lysate preparation protocols.

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