Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase (Ser31) Antibody

We are the original manufacturer of our Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific antibody. It is affinity purified from pooled serum, and great in WB, IHC, and ICC.

Catalog #: p1580-31 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Non-human primate
Applications:
WB 1:1000IHC 1:300-1:1000ICC 1:50-1:300 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
TH
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1580-31, RRID:AB_2492278
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat tyrosine hydroxylase (TH).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat PC12 Cells.

Immunohistochemistry

Rat retina

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

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Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Rat
Western Blot: Human | Mouse | Rat


Immunocytochemistry: Human
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.


Immunocytochemistry: Rat
PMID Dilution Publication
28637871 1:50 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
10936746 1:300 Witkovsky, P, et al. 2000 Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of Chemical Neuroanatomy, 19(2), pp.105-116.


Immunohistochemistry: Rat
PMID Dilution Publication
10936746 1:300 Witkovsky, P, et al. 2000 Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of Chemical Neuroanatomy, 19(2), pp.105-116.


Western Blot: Human
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of Neurochemistry, 79(2), pp.349-360.


Western Blot: Mouse
PMID Dilution Publication
29782882 1:1000 Salvatore, M.F., et al. 2018 Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128.


Western Blot: Rat
PMID Dilution Publication
28637871 1:1000 Jorge-Finnigan, A., et al. 2017 Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
24743698 not listed Izumi, Y, et al. 2014 Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478.
22458761 1:2000 Calipari, E.S., et al. 2014 Methylphenidate and cocaine self‐administration produce distinct dopamine terminal alterations. Addiction Biology, 19(2), pp.145-155.
12124439 1:1500 Jedynak, J.P., et al. 2002 Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of Neurochemistry, 82(2), pp.382-388.
11677263 not listed Salvatore, M.F, et al. 2001 Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of Neurochemistry, 79(2), pp.349-360.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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