Tyrosine Hydroxylase (LNC1) Antibody, Mouse

Non-Ascites Produced LNC1!

Catalog #: 2026-LNC1 Categories: , , , , Datasheet:

$119.00$380.00

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Clone:
LNC1
Formulation:
Protein A Purified Cultured Supernatant
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Most mammals, birds, fish, reptiles, some invertebrates
Applications:
WB 1:1000IHC 1:1000ICC 1:500-1:1000
Host Species:
Mouse monoclonal
Gene Name:
TH
Molecular Weight:
~ 60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2026-LNC1, RRID:AB_2819187
Antigen/Purification: ExpandCollapse

The antigen is Tyrosine Hydroxylase purified from PC12 cells.

Protein A purified tissue culture supernatant.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine.  TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al.,1999).  TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001, Dunkley et al., 2004). LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH.

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM Tris, 50 mM NaCl, 0.065% Sodium Azide pH 7.4

Shipped on blue ice. Recommended that the antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year at -20°C.

General References

Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043.

Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.

Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.

Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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