Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase Antibody, Sheep

Catalog #: 2027-THSHP Categories: , , , Datasheet:

$109.00$365.00

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Excellent dopaminergic neuronal marker for use in IHC!

 

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat, Rabbit
Species Reactivity:
Most mammals, Some invertebrates
Applications:
WB 1:1000IHC 1:1000ICC 1:1000
Species:
Sheep polyclonal
Gene Name:
TH
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2027-THSHP, RRID:AB_2492277
Antigen/Purification: ExpandCollapse

The antigen is a native rat tyrosine hydroxylase, purified from pheochromocytoma.

The antibody is prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.

 

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blotting: Human


Immunohistochemistry: Mouse
PMID Dilution Publication
29584618 not listed Ryu, J.C.., et al. 2018. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. The Journal of Clinical Investigation, May 1;128(5):1772-1786.


Western Blotting: Human
PMID Dilution Publication
12065667 not listed Waymire, J.C. and Haycock, J.W.. 2002. Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells. Journal of Neurochemistry, 81(3), pp.589-593.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tyrosine Hydroxylase Antibody, Sheep
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Click here to view our Western blotting and lysate preparation protocols.
Tyrosine Hydroxylase Antibody, Sheep
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

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