Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase Antibody, Sheep

$365.00

Excellent neuronal marker for use in IHC!

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Most mammals, Some invertebrates
Applications:
WB 1:1,000IF 1:1,000IHC 1:1,000
Species:
Sheep polyclonal
Gene Name:
TH
Molecular Reference:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2027-THSHP, RRID:AB_2492277
Antigen/Purification: ExpandCollapse

The antigen is a native rat tyrosine hydroxylase, purified from pheochromocytoma.

The antibody is prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.

 

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Ryu, J.C., Tooke, K., Malley, S.E., Soulas, A., Weiss, T., Ganesh, N., Saidi, N., Daugherty, S., Saragovi, U., Ikeda, Y. and Zabbarova, I., 2018. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. The Journal of clinical investigation, May 1;128(5):1772-1786. PMID: 29584618

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. PMID: 10936640

Tyrosine Hydroxylase Antibody, Sheep
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Tyrosine Hydroxylase Antibody, Sheep
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

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