Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase Antibody, Rabbit

Catalog #: 2025-THRAB Category: Datasheet:

$345.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Most mammals, Some invertebrates
Applications:
WB 1:1,000IF 1:1000IHC 1:1000
Species:
Rabbit
Molecular Reference:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2025-THRAB, RRID:AB_2492276
Antigen/Purification: ExpandCollapse

The antigen is an SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

de Rus Jacquet, A., Timmers, M., Ma, S.Y., Thieme, A., McCabe, G.P., Vest, J.H.C., Lila, M.A. and Rochet, J.C., 2017. Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology. Feb 15. PMID: 28214539

de Rus Jacquet, A., Tambe, M.A., Ma, S.Y., McCabe, G.P., Vest, J.H.C. and Rochet, J.C., 2017. Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology.  Jan 11. PMID: 28088492

Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. PMID: 11282256

Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. PMID: 11677263

Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.  PMID: 10936746

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.  PMID: 10936640

Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. PMID: 10560033

Xu ZQ, Lew JY, Harada K, Aman K, Goldstein M, Deutch A, Haycock JW, Hokfelt T (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738. PMID: 9483531

Tyrosine Hydroxylase Antibody, Rabbit
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Tyrosine Hydroxylase Antibody, Rabbit
Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons in green and MAP2 (cat. #1100-MAP2) in red. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.

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