Affinity purified from pooled serum. Learn more.

Tyrosine Hydroxylase Antibody, Rabbit

Catalog #: 2025-THRAB Categories: , , , , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Great for use in IHC staining!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Most mammals, Some invertebrates
Applications:
WB 1:1000IHC 1:1000ICC 1:500-1:1000
Species:
Rabbit polyclonal
Gene Name:
TH
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2025-THRAB, RRID:AB_2492276
Antigen/Purification: ExpandCollapse

The antigen is an SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

Synonyms: ExpandCollapse

• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat midbrain cells

Immunohistochemistry

Mouse brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat
Western Blotting: Human |Mouse | Rat


Immunocytochemistry: Rat
PMID Dilution Publication
31034889 1:1000 Sanyal, A., et al. 2019. Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson’s Disease. Journal of molecular biology. May 31;431(12):2266-2282.
29408373 1:1000 Cruz-Hernandez, A., et al. 2018. Selective dopaminergic neurotoxicity of three heterocyclic amine subclasses in primary rat midbrain neurons. Neurotoxicology, 65, pp.68-84.
28214539 1:1000 de Rus Jacquet, A., et al. 2017. Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology. 206:408-425.
28088942 1:500 de Rus Jacquet, A., et al. 2017. Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology, 206, pp.393-407.


Immunohistochemistry: Rat
PMID Dilution Publication
9483531 1:1000 Xu, Z.Q., et al. 1998. Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci . 82:727-738.


Western Blotting: Human
PMID Dilution Publication
14535941 0.5ug-1.5ug/ml Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of neurochemistry, 87(3), pp.574-585.
12065667 not listed Waymire, J.C. and Haycock, J.W., et al. 2002. Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells. Journal of neurochemistry, 81(3), pp.589-593.
11677263 not listed Salvatore, M.F., et al. 2001. Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360.


Western Blotting: Mouse
PMID Dilution Publication
29894763 1:2000 Brindley, R.L., et al. 2018. Adrenal serotonin derives from accumulation by the antidepressant-sensitive serotonin transporter. Pharmacological research. S1043-6618(18)30429-8.
29739866 1:1000 Gowrishanker, R., et al. 2018. Region-Specific Regulation of Presynaptic Dopamine Homeostasis by D2 Autoreceptors Shapes the In Vivo Impact of the Neuropsychiatric Disease-Associated DAT Variant Val559. Journal of Neuroscience, pp.0055-18.


Western Blotting: Rat
PMID Dilution Publication
14535941 0.5ug-1.5ug/ml Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of neurochemistry, 87(3), pp.574-585.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Tyrosine Hydroxylase Antibody, Rabbit
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Click here to view our Western blotting and lysate preparation protocols.

You may also like…