Great for use in IHC staining!
Pooled Serum- Formulation:
- Affinity Purified from Pooled Serum
- Species Tested:
- Human, Mouse, Rat
- Expected Reactivity:
- Most mammals, Some invertebrates
- Applications:
- WB 1:1000IHC 1:1000ICC 1:500-1:1000
- Species:
- Rabbit polyclonal
- Gene Name:
- TH
- Molecular Weight:
- ~60 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 2025-THRAB, RRID:AB_2492276
The antigen is an SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
• Dystonia 14 antibody
• DYT14 antibody
• DYT5b antibody
• EC 1.14.16.2 antibody
• OTTHUMP00000011225 antibody
• OTTHUMP00000011226 antibody
• ple antibody
• Protein Pale antibody
• TH antibody
• The antibody
• TY3H_HUMAN antibody
• TYH antibody
• Tyrosine 3 hydroxylase antibody
• Tyrosine 3 monooxygenase antibody
• Tyrosine 3-hydroxylase antibody
• Tyrosine 3-monooxygenase antibody
• Tyrosine hydroxylase antibody
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Specific Protocols
Immunocytochemistry
Rat midbrain cells
Immunohistochemistry
Mouse brain
Western Blotting
Click here to view our protocols page for Western blotting and lysate preparation.
Product Specific References for Applications and Species
Immunocytochemistry: Rat
Immunohistochemistry: Rat
Western Blotting: Human |Mouse | Rat
| Immunocytochemistry: Rat |
| PMID |
Dilution |
Publication |
| 31034889 |
1:1000 |
Sanyal, A., et al. 2019. Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson’s Disease. Journal of molecular biology. May 31;431(12):2266-2282. |
| 29408373 |
1:1000 |
Cruz-Hernandez, A., et al. 2018. Selective dopaminergic neurotoxicity of three heterocyclic amine subclasses in primary rat midbrain neurons. Neurotoxicology, 65, pp.68-84. |
| 28214539 |
1:1000 |
de Rus Jacquet, A., et al. 2017. Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology. 206:408-425. |
| 28088942 |
1:500 |
de Rus Jacquet, A., et al. 2017. Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology, 206, pp.393-407. |
| Immunohistochemistry: Rat |
| PMID |
Dilution |
Publication |
| 9483531 |
1:1000 |
Xu, Z.Q., et al. 1998. Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci . 82:727-738. |
| Western Blotting: Human |
| PMID |
Dilution |
Publication |
| 14535941 |
0.5ug-1.5ug/ml |
Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of neurochemistry, 87(3), pp.574-585. |
| 12065667 |
not listed |
Waymire, J.C. and Haycock, J.W., et al. 2002. Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells. Journal of neurochemistry, 81(3), pp.589-593. |
| 11677263 |
not listed |
Salvatore, M.F., et al. 2001. Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. |
| Western Blotting: Mouse |
| PMID |
Dilution |
Publication |
| 29894763 |
1:2000 |
Brindley, R.L., et al. 2018. Adrenal serotonin derives from accumulation by the antidepressant-sensitive serotonin transporter. Pharmacological research. S1043-6618(18)30429-8. |
| 29739866 |
1:1000 |
Gowrishanker, R., et al. 2018. Region-Specific Regulation of Presynaptic Dopamine Homeostasis by D2 Autoreceptors Shapes the In Vivo Impact of the Neuropsychiatric Disease-Associated DAT Variant Val559. Journal of Neuroscience, pp.0055-18. |
| Western Blotting: Rat |
| PMID |
Dilution |
Publication |
| 14535941 |
0.5ug-1.5ug/ml |
Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of neurochemistry, 87(3), pp.574-585. |
