Rabbit polyclonal antibody
- 100 µl
- Affinity Purified from Pooled Serum
- Most mammals, Some invertebrates
- WB 1:1,000IF 1:1000IHC 1:1000
- Molecular Reference:
- ~60 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 2025-THRAB, RRID:AB_2492276
The antigen is an SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
de Rus Jacquet, A., Timmers, M., Ma, S.Y., Thieme, A., McCabe, G.P., Vest, J.H.C., Lila, M.A. and Rochet, J.C., 2017. Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology. Feb 15. PMID: 28214539
de Rus Jacquet, A., Tambe, M.A., Ma, S.Y., McCabe, G.P., Vest, J.H.C. and Rochet, J.C., 2017. Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms. Journal of Ethnopharmacology. Jan 11. PMID: 28088492
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. PMID: 11282256
Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. PMID: 11677263
Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. PMID: 10936746
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. PMID: 10936640
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. PMID: 10560033
Xu ZQ, Lew JY, Harada K, Aman K, Goldstein M, Deutch A, Haycock JW, Hokfelt T (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738. PMID: 9483531