Tubulin Antibody, Beta III

Catalog #: 2020-TUB Categories: , , , Datasheet:

$109.00$365.00

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Works great as a neuronal marker in IHC!

 

Formulation:
Protein G Purified
Species Tested:
Mouse, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:10000IHC 1:1000ICC
Species:
Mouse monoclonal
Gene Name:
TUBB3
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2020-TUB, RRID:AB_2492275
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to beta III tubulin.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Tubulin is the major constituent of microtubules, existing as a heterodimer of the ? and ? subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)

Synonyms: ExpandCollapse

• beta 3 tubulin antibody
• beta-4 antibody
• beta III Tubulin
• CDCBM antibody
• CDCBM1 antibody
• CFEOM3 antibody
• CFEOM3A antibody
• FEOM3 antibody
• M(beta)3 antibody
• M(beta)6 antibody
• MC1R antibody
• Neuron specific beta III Tubulin antibody
• Neuron-specific class III beta-tubulin antibody
• QccE-11995 antibody
• QccE-15186 antibody
• TBB3_HUMAN antibody
• Tubb 3 antibody
• TUBB3 antibody
• TUBB4 antibody
• Tubulin beta 3 antibody
• Tubulin beta 3 chain antibody
• Tubulin beta 4 antibody
• Tubulin beta III antibody
• Tubulin beta-3 chain antibody
• Tubulin beta-4 chain antibody
• Tubulin beta-III antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Mouse dentate gyrus
Mouse brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse
Western Blotting: Mouse


Immunocytochemistry: Rat
PMID Dilution Publication
25952507 not listed Cooper, R., et al. 2015. Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238.


Immunohistochemistry: Mouse
PMID Dilution Publication
26526844 not listed Ka, M., et al. 2016. Microtubule-Actin Crosslinking Factor 1 Is Required for Dendritic Arborization and Axon Outgrowth in the Developing Brain. Molecular neurobiology, November, 1-15.


Western Blotting: Mouse
PMID Dilution Publication
26526844 1:8000 Ghersa, F., et al. 2019. Reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55 deficient mice. Molecular human reproduction. 2019 May 9. pii: gaz026.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tubulin Antibody, Beta III
Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.

Click here to view our Western blotting and lysate preparation protocols.

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