Tubulin Antibody, Beta III

Catalog #: 2020-TUB Categories: , , Datasheet:

$365.00

Works great in IHC!

Size:
100 µl
Formulation:
Protein G Purified
Specificity:
Most mammals
Applications:
WB 1:10,000IHC 1:1,000
Species:
Mouse monoclonal
Gene Name:
TUBB3
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2020-TUB, RRID:AB_2492275
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to beta III tubulin.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific References

Ka, M., & Kim, W. Y. (2015). Microtubule-Actin Crosslinking Factor 1 Is Required for Dendritic Arborization and Axon Outgrowth in the Developing Brain. Molecular neurobiology, November, 1-15. PMID: 26526844.

Cooper, R. J., & Spitzer, N. (2015). Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238. PMID: 25952507.

Tubulin Antibody, Beta III
Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
Tubulin Antibody, Beta III
Immunostaining of mouse dentate gyrus showing beta III tubulin in red and nuclei in blue. Photo courtesy of Robert Wine.

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