Affinity purified from pooled serum. Learn more.

Tryptophan Hydroxylase 1 (Ser58) Antibody

Catalog #: p1575-58 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rabbit, Mouse
Expected Reactivity:
Rat, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
TPH1
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1575-58, RRID:AB_2492273
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of tryptophan Hydroxylase 1 (TPH1).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Synonyms: ExpandCollapse

• Indoleacetic acid 5 hydroxylase antibody
• L tryptophan hydroxylase antibody
• MGC119994 antibody
• TPH 1 antibody
• TPH antibody
• TPH1 antibody
• TPH1_HUMAN antibody
• TPRH antibody
• TRPH antibody
• Tryptophan 5 hydroxylase 1 antibody
• Tryptophan 5 monooxygenase 1 antibody
• Tryptophan 5 monooxygenase antibody
• Tryptophan 5-hydroxylase 1 antibody
• Tryptophan 5-monooxygenase 1 antibody
• Tryptophan hydroxylase 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
17727633 1:1000 Kuhn, D.M, et al. 2007 Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. J Neurochem 103(4):1567-73.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tryptophan Hydroxylase Ser58 Antibody
Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of cAMP-dependent protein kinase showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser58.

Click here to view our Western blotting and lysate preparation protocols.

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