Affinity purified from pooled serum. Learn more.

Tryptophan Hydroxylase Antibody

Catalog #: 2015-TPH Category: Datasheet:

$365.00

Sheep polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Human
Applications:
WB 1:1000IHC 1:1000
Species:
Sheep
Gene Name:
TPH1
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2015-TPH, RRID:AB_2492271
Antigen/Purification: ExpandCollapse

The antigen is a recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

The antibody is prepared from pooled sheep serum by affinity purification using a column to which the recombinant protein was coupled.

Biological Significance: ExpandCollapse

Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Kuhn, D.M., Sakowski, S.A., Geddes, T.J., Wilkerson, C. and Haycock, J.W., 2007. Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine‐19 as the substrate site for calcium, calmodulin‐dependent protein kinase II. Journal of neurochemistry, 103(4), pp.1567-1573. PMID: 17727633

Haycock, J.W., Kumer, S.C., Lewis, D.A., Vrana, K.E. and Stockmeier, C.A., 2002. A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. Journal of neuroscience methods, 114(2), pp.205-212. PMID: 11856572
Tryptophan Hydroxylase Antibody
Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein.

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