Sheep polyclonal antibodyPooled Serum
- 100 µl
- Affinity Purified from Pooled Serum
- Rat, Human
- WB 1:1000IHC 1:1000
- Gene Name:
- Molecular Reference:
- ~55 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 2015-TPH, RRID:AB_2492271
The antigen is a recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
The antibody is prepared from pooled sheep serum by affinity purification using a column to which the recombinant protein was coupled.
Biological Significance: ExpandCollapse
Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).