Affinity purified from pooled serum. Learn more.

Tryptophan Hydroxylase 2 (Ser19) Antibody

Catalog #: p1575-19 Categories: , Datasheet:

$119.00$380.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
TPH2
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1575-19, RRID:AB_2492272
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).

Synonyms: ExpandCollapse

• MGC138871 antibody
• ADHD7 antibody
• FLJ37295 antibody
• MGC138872 antibody
• Neuronal tryptophan hydroxylase antibody
• NTPH antibody
• TPH 2 antibody
• Tph2 antibody
• TPH2 antibody
• TPH2_HUMAN antibody
• Tryptophan 5-hydroxylase 2 antibody
• Tryptophan 5-monooxygenase 2 antibody
• Tryptophan hydroxylase 2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
17727633 1:1000 Kuhn, D.M, et al. 2007 Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. J Neurochem 103(4):1567-73.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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