Affinity purified from pooled serum. Learn more.

Tryptophan Hydroxylase 2 (Ser19) Antibody

Catalog #: p1575-19 Category: Datasheet:

$365.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Bovine, Zebra fish
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
TPH2
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1575-19, RRID:AB_2492272
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017.

Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823.

Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

McKinney J, Knappskog PM, Haavik J (2005) Different properties of the central and peripheral forms of human tryptophan hydrpxylase. J Neurochem 92(2):311-20.

Kuhn DM, Sakowski SA, Geddes TJ, Wilkerson C, Haycock JW (2007) Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. J Neurochem 103(4):1567-73.

Tryptophan Hydroxylase 2 Ser19 Antibody
Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of Ca2+/calmodulin dependent kinase II showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19.

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