Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Xenopus, Bovine, Non-human primate, Zebra fish, Sheep, Canine
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~25 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p2010-43, RRID:AB_2492269
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser43 of mouse troponin I, cardiac (cTnI).
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKCmediated depression. (Montgomery et al, 2002).