Affinity purified from pooled serum. Learn more.

Troponin I, cardiac (Ser150) Antibody

Catalog #: p2010-150 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Non-human primate
Applications:
WB 1:1,000
Species:
Rabbit Polyclonal
Molecular Reference:
~25 kDa
Cite This Antibody:
PhosphoSolutions Cat# p2010-150, RRID:AB_2492270
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser150 of mouse troponin I, cardiac (cTnI).

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Thoemmes, S. F., Stutzke, C. A., Du, Y., Browning, M. D., Buttrick, P. M., & Walker, L. A. (2014). Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of immunological methods, 403(1), 66-71. PMID: 24291343

Troponin I, cardiac Ser150 Antibody
Western blot of mouse heart lysate showing specific immunolabeling of ~25 kDa cardiac troponin I protein phosphorylated at Ser150 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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