Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Non-human primate
- WB 1:1,000
- Rabbit Polyclonal
- Molecular Reference:
- ~25 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p2010-150, RRID:AB_2492270
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser150 of mouse troponin I, cardiac (cTnI).
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).