Our Troponin I (cardiac) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects Troponin I (cardiac) and is neat serum. It is great for use in WB, IHC.
Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein.
Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser-43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr-144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).
Neat Serum
Polyclonal
IgG
IHC, WB
Rabbit
TNNI3
25 kDa
Fusion protein of complete mouse cardiac troponin 1 holoprotein.
Mouse, Rat
AB_2492267
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat Serum
Neat serum
WB: 1:2000
Unconjugated
Specific for endogenous levels of the ~25 kDa cardiac troponin I protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody
UniProt (Immunogen Species): P48787
21954
Product Specific References for Applications and Species
Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.
Walker, L.A., et al. 2013. Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), pp.H1644-H1650.
Yusifova, M., et al. 2023. Voluntary Wheel Running Exercise Does Not Attenuate Circadian and Cardiac Dysfunction Caused by Conditional Deletion of Bmal1. Journal of Biological Rhythms, 7487304231152398.
Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.
