Thyroid Hormone Receptor Antibody, α1/α2-Isotype

Our Thyroid Hormone Receptor α1/α2-Isotype mouse monoclonal antibody is Protein G purified. It is excellent for use in WB.

Catalog #: 1210-TRa Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Protein G Purified
Species Tested:
Human, Rat
Expected Reactivity:
Canine, Mouse, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
THRA
Molecular Weight:
~50 kDa and ~58 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1210-TRa, RRID:AB_2492260
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, α12-isotype.

The antibody is prepared from tissue culture supernatant by ammonium sulfate precipitation followed by  purification on a Protein G column.

Biological Significance: ExpandCollapse

Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRα1, TRα2, TRβ1 and TRβ2. TRα1, TRβ1 and TRβ2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).

Synonyms: ExpandCollapse

• c-erbA-1 antibody
• C-erbA-alpha antibody
• EAR-7 antibody
• ERBA 1 antibody
• ERBA antibody
• ERBA related 7 antibody
• Nuclear receptor subfamily 1 group A member 1 antibody
• THA_HUMAN antibody
• THRA antibody
• Thra1 antibody
• Thra2 antibody
• Thyroid hormone receptor alpha 1 antibody
• Thyroid hormone receptor alpha 2 antibody
• Thyroid hormone receptor alpha antibody
• TR alpha 1 antibody
• TR alpha 2 antibody
• Tra1 antibody
• Triiodothyronine receptor antibody
• V-erbA-related protein 7 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Ambrogini, P., Cuppini, R., Ferri, P., Mancini, C., Ciaroni, S., Voci, A., Gerdoni, E. and Gallo, G., 2005. Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology, 81(4), pp.244-253. PMID: 16113586

Chan, S. and Kilby, M.D., 2000. Thyroid hormone and central nervous system development. The Journal of Endocrinology, 165(1), p.1-8. PMID: 10750030

Constantinou, C., Margarity, M. and Valcana, T., 2005. Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain. Molecular and Cellular Biochemistry, 278(1-2), pp.93-100. PMID: 16180094

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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