Thyroid Hormone Receptor Antibody, α1/α2-Isotype

Catalog #: 1210-TRa Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Reactivity:
Rat, Mouse, Human, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Mouse
Gene Name:
THRA
Molecular Weight:
~50 kDa and ~58 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1210-TRa, RRID:AB_2492260
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, ?1/?2-isotype.

The antibody is prepared from tissue culture supernatant by ammonium sulfate precipitation followed by  purification on a Protein G column.

Biological Significance: ExpandCollapse

Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TR?1, TR?2, TR?1 and TR?2. TR?1, TR?1 and TR?2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).

Synonyms: ExpandCollapse

• c-erbA-1 antibody
• C-erbA-alpha antibody
• EAR-7 antibody
• ERBA 1 antibody
• ERBA antibody
• ERBA related 7 antibody
• Nuclear receptor subfamily 1 group A member 1 antibody
• THA_HUMAN antibody
• THRA antibody
• Thra1 antibody
• Thra2 antibody
• Thyroid hormone receptor alpha 1 antibody
• Thyroid hormone receptor alpha 2 antibody
• Thyroid hormone receptor alpha antibody
• TR alpha 1 antibody
• TR alpha 2 antibody
• Tra1 antibody
• Triiodothyronine receptor antibody
• V-erbA-related protein 7 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Ambrogini P, Cuppini R, Ferri P, Mancini C, Ciaroni S, Voci A, Gerdoni E, Gallo G (2005) Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology 81:244-253.

Chan S, Kilby MD (2000) Thyroid hormone and central nervous system development. J Endocrinol 165:1-8.

Constantinou C, Margarity M, Valcana T (2005) Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain. Mol Cell Biochem 278:93-100.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Thyroid Hormone Receptor Antibody, α1/α2-Isotype
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~50 kDa TR-?1 and the ~58 kDa TR-?2 protein.

You may also like…