TFAM (Transcription Factor A, mitochondrial) Antibody, Human

Our human TFAM rabbit polyclonal antibody is formulated as neat serum. It is excellent for use in WB.

Catalog #: 1999-hTFAM Categories: , , Datasheet:

$109.00$325.00

  • SizePrice
Clear
Formulation:
Neat serum
Species Tested:
Human
Applications:
WB 1:2000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
TFAM
Molecular Weight:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1999-hTFAM, RRID:AB_2571641
Antigen/Purification: ExpandCollapse

The antigen is a native recombinant human TFAM protein with c-terminal 6-his tag.

The antibody is serum.

Biological Significance: ExpandCollapse

Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).

Synonyms: ExpandCollapse

• Tanscription factor 6-like 1 antibody
• Mitochondrial transcription factor 1 antibody
• mitochondrial transcription factor A antibody
• MtTF1 antibody
• mtTFA antibody
• TCF 6 antibody
• TCF-6 antibody
• TCF6 antibody
• TCF6L1 antibody
• TCF6L2 antibody
• TCF6L3 antibody
• TFAM antibody
• TFAM_HUMAN antibody
• Transcription factor 6 antibody
• Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
• Transcription factor 6 like 2 antibody
• Transcription factor 6-like 2 antibody
• transcription factor 6-like 3 antibody
• Transcription factor A, mitochondrial antibody
• Transcription factor A, mitochondrial antibody
• Transcription factor A, mitochondrial precursor antibody

Storage

100 µl neat serum. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Hayashi, Y., Yoshida, M., Yamato, M., Ide, T., Wu, Z., Ochi-Shindou, M., Kanki, T., Kang, D., Sunagawa, K., Tsutsui, H. and Nakanishi, H., 2008. Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice. Journal of Neuroscience, 28(34), pp.8624-8634. PMID: 18716221

Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T. and Abe, K., 2012. Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice. Journal of Neuroscience Research, 90(6), pp.1200-1208. PMID: 22354563

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…