Tau Antibody

Excellent neuronal axon marker! Our Tau chicken polyclonal antibody is total IgY. It is excellent for use in WB, ICC, and IHC.

Catalog #: 1998-TAU Categories: , , , , Datasheet:
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$115.00 – $335.00

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Clear
Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Applications:
WB 1:10000IHC 1:1000ICC 1:1000-1:2000
Host Species:
Chicken Polyclonal
Gene Name:
MAPT
Molecular Weight:
~48 kDa, ~65 kDa & ~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1998-TAU, RRID:AB_2492256
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human tau expressed in and purified from E. Coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy.

Synonyms: ExpandCollapse

• AI413597 antibody
• AW045860 antibody
• DDPAC antibody
• FLJ31424 antibody
• FTDP 17 antibody
• G protein beta1/gamma2 subunit interacting factor 1 antibody
• MAPT antibody
• MAPTL antibody
• MGC134287 antibody
• MGC138549 antibody
• MGC156663 antibody
• Microtubule associated protein tau antibody
• Microtubule associated protein tau isoform 4 antibody
• Microtubule-associated protein tau antibody
• MSTD antibody
• Mtapt antibody
• MTBT1 antibody
• MTBT2 antibody
• Neurofibrillary tangle protein antibody
• Paired helical filament tau antibody
• Paired helical filament-tau antibody
• PHF tau antibody
• PHF-tau antibody
• PPND antibody
• PPP1R103 antibody
• Protein phosphatase 1, regulatory subunit 103 antibody
• pTau antibody
• RNPTAU antibody
• TAU antibody
• TAU_HUMAN antibody
• Tauopathy and respiratory failure, included antibody

Storage

100 µl liquid containing 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat hippocampal neurons
E20 Rat cortical neuron and glial cultures

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Human

Immunohistochemistry: Human
PMIDDilutionPublication
333829791:1000 Zhang, K.Y., et al. 2020 Role of the Internal Limiting Membrane in Structural Engraftment and Topographic Spacing of Transplanted Human Stem Cell-Derived Retinal Ganglion Cells. Stem Cell Reports,16(1),149-167.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tau Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~48 kDa, ~65 kDa & ~75 kDa tau isoforms.

Click here to view our Western blotting and lysate preparation protocols.
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-TAU antibody (catalog #1998-TAU, green, 1:2000) and anti-MAP2 (red). The blue is DAPI staining nuclear DNA. Anti-TAU labels perikarya, dendrites, axons of neurons while anti-MAP2 only labels dendrites and perikarya of neurons. Where they overlap they appear orange-yellow.

Download the protocol used for this image:

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