Affinity purified from pooled serum. Learn more.

TAO2 (Ser181) Antibody

We are the original manufacturer of our TAO (Ser181) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Works great in WB!

Catalog #: p275-181 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Avian, Canine, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
TAOK2
Molecular Weight:
~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# p275-181, RRID:AB_2492255
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Synonyms: ExpandCollapse

• 1110033K02Rik antibody
• B230344N16 antibody
• hKFC C antibody
• hKFC-C antibody
• KIAA0881 antibody
• Kinase from chicken homolog C antibody
• MAP3K17 antibody
• mKIAA0881 antibody
• Prostate derived STE20 like kinase 1 antibody
• Prostate derived STE20 like kinase PSK antibody
• Prostate derived sterile 20 like kinase 1 antibody
• Prostate-derived STE20-like kinase 1 antibody
• PSK 1 antibody
• PSK antibody
• PSK-1 antibody
• PSK1 antibody
• PSK1 beta antibody
• Serine/threonine protein kinase TAO2 antibody
• Serine/threonine-protein kinase TAO2 antibody
• TAO 1 antibody
• TAO 2 antibody
• TAO kinase 2 antibody
• TAO1 antibody
• TAO2 antibody
• TAOK2 antibody
• TAOK 2 antibody
• Taok2 antibody
• TAOK2_HUMAN antibody
• Thousand and one amino acid protein 2 antibody
• Thousand and one amino acid protein kinase antibody
• UNQ2971/PRO7431 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Chen, Z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G. and Cobb, M.H., 2003. TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. Journal of Biological Chemistry, 278(25), pp.22278-22283. PMID: 12665513

Chen, Z. and Cobb, M.H., 2001. Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. Journal of Biological Chemistry, 276(19), pp.16070-16075. PMID: 11279118

Chen, Z., Hutchison, M. and Cobb, M.H., 1999. Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain. Journal of Biological Chemistry, 274(40), pp.28803-28807. PMID: 10497253

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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