Affinity purified from pooled serum. Learn more.

TAO2 (Ser181) Antibody

Catalog #: p275-181 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Zebra fish, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
TAOK2
Molecular Weight:
~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# p275-181, RRID:AB_2492255
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Synonyms: ExpandCollapse

• 1110033K02Rik antibody
• B230344N16 antibody
• hKFC C antibody
• hKFC-C antibody
• KIAA0881 antibody
• Kinase from chicken homolog C antibody
• MAP3K17 antibody
• mKIAA0881 antibody
• Prostate derived STE20 like kinase 1 antibody
• Prostate derived STE20 like kinase PSK antibody
• Prostate derived sterile 20 like kinase 1 antibody
• Prostate-derived STE20-like kinase 1 antibody
• PSK 1 antibody
• PSK antibody
• PSK-1 antibody
• PSK1 antibody
• PSK1 beta antibody
• Serine/threonine protein kinase TAO2 antibody
• Serine/threonine-protein kinase TAO2 antibody
• TAO 1 antibody
• TAO 2 antibody
• TAO kinase 2 antibody
• TAO1 antibody
• TAO2 antibody
• TAOK2 antibody
• TAOK 2 antibody
• Taok2 antibody
• TAOK2_HUMAN antibody
• Thousand and one amino acid protein 2 antibody
• Thousand and one amino acid protein kinase antibody
• UNQ2971/PRO7431 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH (2003) TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. J Biol Chem 278:22278-22283.

Chen Z, Cobb, MH (2001) Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. J Biol Chem 276:16070-16075.

Chen Z, Hutchison M, Cobb MH (1999) Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain. J Biol Chem 274:28803-28807.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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TAO2 Ser 181 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at Ser181 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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