Syntaxin 1A Antibody

Catalog #: 1990-STX Categories: , , , Datasheet:

$109.00$365.00

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Great for use in IHC!

Formulation:
Protein G Purified
100% Homology:
Rat, Human, Porcine
Applications:
WB 1:1,000IHC 1:100
Species:
Mouse monoclonal
Gene Name:
STX1A
Molecular Reference:
~36 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1990-STX, RRID:AB_2492254
Antigen/Purification: ExpandCollapse

The antigen is a synaptic immunoprecipitate (crude) from human brain.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Khvotchev M, Dulubova I, Sun J, Dai H, Rizo J, Südhof TC (2007) Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus. J Neurosci. 2007 Nov 7;27(45):12147-55

Castillo MA, Ghose S, Tamminga CA, Ulery-Reynolds PG (2010) Deficits in syntaxin 1 phosphorylation in schizophrenia prefrontal cortex. Biol Psychiatry. 67(3):208-16

Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Research 609, 9-20 (1993).

Syntaxin 1A Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.

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