Syntaxin 1A Antibody

Our Syntaxin 1A mouse monoclonal antibody is Protein G purified. It is excellent for use in WB and IHC!

Catalog #: 1990-STX Categories: , , , , Datasheet:
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$119.00 – $380.00

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Formulation:
Protein G Purified
Species Tested:
Hamster, Human, Porcine, Rat
Applications:
WB 1:1000IHC 1:100 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
STX1A
Molecular Weight:
~36 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1990-STX, RRID:AB_2492254
Antigen/Purification: ExpandCollapse

The antigen is a synaptic immunoprecipitate (crude) from human brain.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).

Synonyms: ExpandCollapse

• HPC 1 antibody
• Neuron specific antigen HPC1 antibody
• Neuron-specific antigen HPC-1 antibody
• OTTHUMP00000174615 antibody
• OTTHUMP00000174616 antibody
• OTTHUMP00000174617 antibody
• OTTHUMP00000174618 antibody
• P35-1 antibody
• STX1 antibody
• STX1A antibody
• STX1A_HUMAN antibody
• SYN1A antibody
• Syntaxin 1A (brain) antibody
• Syntaxin 1A brain antibody
• Syntaxin-1A antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID Dilution Publication
30640971 1:100 Blandford, S.N., et al. 2019 Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative Ophthalmology and Visual Science, 60(1), pp.183-191.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Syntaxin 1A Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.

Click here to view our Western blotting and lysate preparation protocols.

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