Affinity purified from pooled serum. Learn more.

Synaptotagmin (Ser309) Antibody

We are the original manufacturer of our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Great for WB and ICC.

Catalog #: p1570-309 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Bovine, Canine, Chicken, Human, Mouse, Non-human primate, Zebra fish
Applications:
WB 1:1000ICC 1:400 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
SYT1
Molecular Weight:
~60 kDa - 62 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1570-309, RRID:AB_2492253
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Synonyms: ExpandCollapse

• DKFZp781D2042 antibody
• FLJ42519 antibody
• P65 antibody
• SVP65 antibody
• synaptotagmin 1 antibody
• Synaptotagmin I antibody
• SYT antibody
• SYT1 antibody
• SytI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Rat


Western Blot: Rat
PMIDDilutionPublication
18519737not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. The Journal of Cell Biology, 181(5), pp.831-846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…