Affinity purified from pooled serum. Learn more.

Synaptotagmin Antibody

We are the original manufacturer of our Synaptotagmin rabbit polyclonal antibody, affinity purified from pooled serum. Works great in WB!

Catalog #: 1975-STG Categories: , , , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Chicken, Human, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
SYT1
Molecular Weight:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1975-STG, RRID:AB_2492251
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues within the synaptotagmin I protein.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Synonyms: ExpandCollapse

• DKFZp781D2042 antibody
• FLJ42519 antibody
• P65 antibody
• SVP65 antibody
• synaptotagmin 1 antibody
• Synaptotagmin I antibody
• SYT antibody
• SYT1 antibody
• SytI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western blot: Mouse
PMID Dilution Publication
32611447 1:1000 White, J.A., et al. 2020. Excess Rab4 rescues synaptic and behavioral dysfunction caused by defective HTT-Rab4 axonal transport in Huntington’s disease. Acta Neuropathologica Communications, 8(1), pp.1-22.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…