Affinity purified from pooled serum. Learn more.

Synaptotagmin Antibody

Catalog #: 1975-STG Categories: , , , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
SYT1
Molecular Weight:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1975-STG, RRID:AB_2492251
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues within the synaptotagmin I protein.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Synonyms: ExpandCollapse

• DKFZp781D2042 antibody
• FLJ42519 antibody
• P65 antibody
• SVP65 antibody
• synaptotagmin 1 antibody
• Synaptotagmin I antibody
• SYT antibody
• SYT1 antibody
• SytI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Littleton JT, Bellen HJ (1995) Synaptotagmin controls and modulates synaptic vesicle fusion in a Ca(2+) dependent manner. Trends Neurosci. Apr;18(4):177-83.

Zhang X, Kim-Miller MJ, Fukuda M, Kowalchyk JA, Martin TF (2002) Ca2+ dependent synaptotagmin binding to SNAP25 is essential for Ca2+ triggered exocytosis. Neuron May 16;34(4):599-611.

Poskanzer KE, Fetter RD, Davis GW (2006) Discrete residues in the c(2)b domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Neuron Apr 6;50(1):49-62.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Synaptotagmin Antibody
Western blot of rat cortical lysate showing specific labeling of the ~65 kDa synaptotagmin protein.

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