Affinity purified from pooled serum. Learn more.

Synaptotagmin Antibody

Catalog #: 1975-STG Categories: , , , , Datasheet:

$119.00$380.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Chicken, Human, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
SYT1
Molecular Weight:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1975-STG, RRID:AB_2492251
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues within the synaptotagmin I protein.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Synonyms: ExpandCollapse

• DKFZp781D2042 antibody
• FLJ42519 antibody
• P65 antibody
• SVP65 antibody
• synaptotagmin 1 antibody
• Synaptotagmin I antibody
• SYT antibody
• SYT1 antibody
• SytI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Littleton, J.T. and Bellen, H.J., 1995. Synaptotagmin controls and modulates synaptic-vesicle fusion in a Ca2+-dependent manner. Trends in Neurosciences, 18(4), pp.177-183. PMID: 7778189

Zhang, X., Kim-Miller, M.J., Fukuda, M., Kowalchyk, J.A. and Martin, T.F., 2002. Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosis. Neuron, 34(4), pp.599-611. PMID: 12062043

Poskanzer, K.E., Fetter, R.D. and Davis, G.W., 2006. Discrete residues in the C2B domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. Neuron, 50(1), pp.49-62. PMID: 16600855

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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