Affinity purified from pooled serum. Learn more.

Synaptotagmin Antibody

Catalog #: 1975-STG Categories: , , , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
SYT1
Molecular Reference:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1975-STG, RRID:AB_2492251
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues within the synaptotagmin I protein.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Littleton JT, Bellen HJ (1995) Synaptotagmin controls and modulates synaptic vesicle fusion in a Ca(2+) dependent manner. Trends Neurosci. Apr;18(4):177-83.

Zhang X, Kim-Miller MJ, Fukuda M, Kowalchyk JA, Martin TF (2002) Ca2+ dependent synaptotagmin binding to SNAP25 is essential for Ca2+ triggered exocytosis. Neuron May 16;34(4):599-611.

Poskanzer KE, Fetter RD, Davis GW (2006) Discrete residues in the c(2)b domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Neuron Apr 6;50(1):49-62.

Synaptotagmin Antibody
Western blot of rat cortical lysate showing specific labeling of the ~65 kDa synaptotagmin protein.

You may also like…