Synaptobrevin (VAMP) Antibody

Catalog #: 1933-SYB Categories: , Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
100% Homology:
Rat, Mouse, Human, Bovine, Porcine, Rabbit
Applications:
WB 1:1,000IHC 1:100
Species:
Mouse
Gene Name:
VAMP1/2
Molecular Reference:
~16 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1933-SYB, RRID:AB_2492248
Antigen/Purification: ExpandCollapse

The antigen is a synaptic immunoprecipitate (crude) from human brain.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive deficits in Alzheimer’s disease (Sze et al., 2000).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Deák F, Schoch S, Liu X, Südhof TC, Kavalali ET (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nat Cell Biol. 2004 Nov;6(11):1102-8.

Sze CI, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ (2000) Selective regional loss of exocytotic presynaptic vesicle proteins in Alzheimer’s disease brains. J Neurol Sci.175(2):81-90.

Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex (1993). Brain Research 609, 9-20.

Synaptobrevin (VAMP) Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein.

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