Affinity purified from pooled serum. Learn more.

Synapsin I (Ser9) Antibody

Catalog #: p1560-9 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat, Zebrafish
Species Reactivity:
Human, Xenopus, Bovine
Applications:
WB 1:1000ICC 1:500 Don't see your application?
Species:
Rabbit
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1560-9, RRID:AB_2492244
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of synapsin I.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Western Blot: Mouse | Zebrafish


Immunocytochemistry: Mouse
PMID Dilution Publication
25609616 1:200 Shulman, Y., et al. 2015 ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. Journal of Neuroscience, 35(3), pp.985-998.


Western Blot: Mouse
PMID Dilution Publication
26514267 1:1000 Benleulmi-Chaachoua, A., et al. 2016 Protein interactome mining defines melatonin MT 1 receptors as integral component of presynaptic protein complexes of neurons. Journal of pineal research, 60(1), pp.95-108.


Western Blot: Zebrafish
PMID Dilution Publication
20097902 not listed Shimomura, S., et al. 2010 Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant. The Journal of Biochemistry, 147(5), pp.711-722.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Synapsin I Ser9 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser9 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Click here to view our Western blotting and lysate preparation protocols.
Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (catalog # p1560-9, 1:500, red). Cells and photo courtesy of QBMCellScience.

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