Affinity purified from pooled serum. Learn more.

Synapsin I (Ser9) Antibody

We are the original manufacturer of our Synapsin I (Ser9) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. It is excellent for use in WB and ICC.

Catalog #: p1560-9 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat, Zebrafish
Expected Reactivity:
Human, Xenopus, Bovine
Applications:
WB 1:1000ICC 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1560-9, RRID:AB_2492244
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of synapsin I.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Western Blot: Mouse | Zebrafish


Immunocytochemistry: Mouse
PMIDDilutionPublication
256096161:200Shulman, Y., et al. 2015 ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. Journal of Neuroscience, 35(3), pp.985-998.


Western Blot: Mouse
PMIDDilutionPublication
265142671:1000Benleulmi-Chaachoua, A., et al. 2016 Protein interactome mining defines melatonin MT 1 receptors as integral component of presynaptic protein complexes of neurons. Journal of Pineal Research, 60(1), pp.95-108.


Western Blot: Zebrafish
PMIDDilutionPublication
20097902not listedShimomura, S., et al. 2010 Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant. The Journal of Biochemistry, 147(5), pp.711-722.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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