Affinity purified from pooled serum. Learn more.

Synapsin I (Ser603) Antibody

Catalog #: p1560-603 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1560-603, RRID:AB_2492247
Antigen/Purification: ExpandCollapse

The antigen is Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser603 of synapsin I.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western Blot: Human
PMID Dilution Publication
23255606 1:10,000 Magupalli, V.G., et al. 2013 Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.


Western Blot: Mouse
PMID Dilution Publication
19609731 1:1000 Takahashi, E., et al. 2010 Interaction Between Ca v 2.1 α 1 and CaMKII in Ca v 2.1 α 1 Mutant Mice, Rolling Nagoya. Journal of molecular neuroscience, 41(2), pp.223-229.


Western Blot: Rat
PMID Dilution Publication
18519737 not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 – 846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Synapsin I Ser603 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Click here to view our Western blotting and lysate preparation protocols.

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