Affinity purified from pooled serum. Learn more.

Synapsin I Antibody

Catalog #: 1926-SYNP Categories: , , , Datasheet:

$109.00$365.00

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Works great in IHC!!!

 

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse, Human
Applications:
WB 1:1000IP 1 µl per 200 µg lysateICC 1:1000-1:2000 Don't see your application?
Species:
Rabbit Polyclonal
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1926-SYNP, RRID:AB_2492242
Antigen/Purification: ExpandCollapse

The antigen is a native protein purified from bovine brain.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Western Blot: Human | Mouse | Rat


Immunocytochemistry: Mouse
PMID Dilution Publication
26706461 1:2000 Wahba, G., et al. 2016 A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of neuroscience methods, 261, pp.110-116..


Western Blot: Human
PMID Dilution Publication
23255606 1:10,000 Magupalli, V.G., et al. 2013 Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), pp.4637-4648.


Western Blot: Mouse
PMID Dilution Publication
27335405 not listed Song, S.H., et al. 2016 Synapsin isoforms regulating GABA release from hippocampal interneurons. Journal of Neuroscience, 36(25), pp.6742-6757.


Western Blot: Rat
PMID Dilution Publication
18819923 1:1000 Davies, K.D., et al. 2008 Long term synaptic depression that is associated with GluR1 dephosphorylation but not α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization. Journal of Biological Chemistry, 283(48), pp.33138-33146.
18819923 not listed Grosshans, D.R., et al. 2002 LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature neuroscience, Jan;5(1):27-33.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Synapsin I Antibody
Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

Click here to view our Western blotting and lysate preparation protocols.

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