Synapsin I Antibody, 50 µl

Catalog #: 1927-SYNP Categories: , , , Datasheet:

$310.00

Rabbit polyclonal antibody, neat serum

Size:
50 µl
Formulation:
Neat serum
Species Tested:
Rat, Mouse, Human
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1927-SYNP, RRID:AB_2492243
Antigen/Purification: ExpandCollapse

The antigen is a native protein purified from bovine brain.

The antibody is unpurified neat serum.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in the brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

50 µl neat serum.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372- 4380. PMID: 12040043

Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ** (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953. PMID: 11588168

Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ**, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437. PMID: 11976703

Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162. PMID: 2498469

Nayak AS, Moore CI, Browning MD** (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456. PMID: 8986832

Stone LM, Browning MD**, Finger TE (1994) Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci 14:301-309. PMID: 8283238

**Dr. Michael Browning and Dr. Andrew Czernik, co-authors of the cited papers above are the President and Chief Scientific Officer of PhosphoSolutions and two of its founders.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Synapsin I Antibody, 50 µl
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

Click here to view our Western blotting and lysate preparation protocols.

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