Synapsin I Antibody, 50 µl

Catalog #: 1927-SYNP Categories: , , , Datasheet:

$310.00

Rabbit polyclonal antibody, neat serum

Size:
50 µl
Formulation:
Neat serum
100% Homology:
Rat, Mouse, Human
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
SYN1
Molecular Reference:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1927-SYNP, RRID:AB_2492243
Antigen/Purification: ExpandCollapse

The antigen is a native protein purified from bovine brain.

The antibody is unpurified neat serum.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in the brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Storage

50 μl neat serum. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372- 4380.

Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.

Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.

Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.

Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

Stone LM, Browning MD, Finger TE (1994) Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci 14:301-309.

Note: Dr. Michael Browning and Dr. Andrew Czernik, co-authors of the cited papers are the President and Chief Scientific Officer of PhosphoSolutions and two of its founders.

Synapsin I Antibody, 50 µl
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

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