Affinity purified from pooled serum. Learn more.

Synapsin I Antibody, 10 µg

Catalog #: 1925-SYNP Categories: , , , Datasheet:

$365.00

Excellent pre-synaptic marker for use in IHC!

Pooled Serum
Size:
10 µg
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Rat, Mouse, Human
Applications:
WB 1:1000 IF 1:1,000 IHC 1:1,000 IP 1 µg per 200 µg lysate
Species:
Rabbit polyclonal
Gene Name:
SYN1
Molecular Reference:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1925-SYNP, RRID:AB_2492241
Antigen/Purification: ExpandCollapse

The antigen is a native protein purified from bovine brain.

The antibody prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Storage

10 μg antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquoted and stored at -20° C. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Song, S. H., & Augustine, G. J. (2016). Synapsin isoforms regulating GABA release from hippocampal interneurons. The Journal of Neuroscience, 36(25), 6742-6757. PMID: 27335405

Wahba, G., Hebert, A. E., Grynspan, D., Staines, W., & Schock, S. (2016). A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of neuroscience methods, 261, 110-116. PMID: 26706461

Magupalli, V. G., Mochida, S., Yan, J., Jiang, X., Westenbroek, R. E., Nairn, A. C., Scheuer, T. & Catterall, W. A. (2013). Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.  PMID: 23255606

Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA)Receptor Internalization J. Biol. Chem., 283: 33138 – 33146. PMID: 18819923

Synapsin I Antibody, 10 µg
Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

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