Excellent pre-synaptic marker for use in IHC!
- 10 µg
- Affinity Purified from Pooled Serum
- Species Tested:
- Rat, Mouse, Human
- WB 1:1000IP 1 µg per 200 µg lysateICC 1:1000-1:2000 Don't see your application?
- Rabbit polyclonal
- Gene Name:
- Molecular Weight:
- ~78 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1925-SYNP, RRID:AB_2492241
The antigen is a native protein purified from bovine brain.
The antibody prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody
10 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.
The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Product Specific Protocols
Click here to view our protocols page for Western blotting and lysate preparation.
Product Specific References for Applications and Species
Western Blot: Human | Mouse | Rat
||Wahba, G., et al. 2016 A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of neuroscience methods, 261, pp.110-116..
|Western Blot: Human
||Magupalli, V.G., et al. 2013 Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), pp.4637-4648.
|Western Blot: Mouse
||Song, S.H., et al. 2016 Synapsin isoforms regulating GABA release from hippocampal interneurons. Journal of Neuroscience, 36(25), pp.6742-6757.
|Western Blot: Rat
||Davies, K.D., et al. 2008 Long term synaptic depression that is associated with GluR1 dephosphorylation but not α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization. Journal of Biological Chemistry, 283(48), pp.33138-33146.
||Grosshans, D.R., et al. 2002 LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature neuroscience, Jan;5(1):27-33.