Affinity purified from pooled serum. Learn more.

Synapsin I Antibody, 10 µg

Catalog #: 1925-SYNP Categories: , , , Datasheet:

$365.00

Excellent pre-synaptic marker for use in IHC!

Pooled Serum
Size:
10 µg
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human
Applications:
WB 1:1000IF 1:1000IHC 1:10IP 1 µg per 200 µg lysate
Species:
Rabbit polyclonal
Gene Name:
SYN1
Molecular Reference:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1925-SYNP, RRID:AB_2492241
Antigen/Purification: ExpandCollapse

The antigen is a native protein purified from bovine brain.

The antibody prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Synonyms: ExpandCollapse

• Brain protein 4.1 antibody
• SYN 1 antibody
• SYN 1a antibody
• SYN 1b antibody
• SYN I antibody
• SYN1 antibody
• SYN1_HUMAN antibody
• SYN1a antibody
• SYN1b antibody
• Synapsin 1 antibody
• Synapsin I antibody
• Synapsin-1 antibody
• Synapsin1 antibody
• SynapsinI antibody
• SYNI antibody

Storage

10 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.

The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific References

Song, S. H., & Augustine, G. J. (2016). Synapsin isoforms regulating GABA release from hippocampal interneurons. The Journal of Neuroscience, 36(25), 6742-6757. PMID: 27335405

Wahba, G., Hebert, A. E., Grynspan, D., Staines, W., & Schock, S. (2016). A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of neuroscience methods, 261, 110-116. PMID: 26706461

Magupalli, V. G., Mochida, S., Yan, J., Jiang, X., Westenbroek, R. E., Nairn, A. C., Scheuer, T. & Catterall, W. A. (2013). Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.  PMID: 23255606

Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA)Receptor Internalization J. Biol. Chem., 283: 33138 – 33146. PMID: 18819923

Reviews

There are no reviews yet.

Be the first to review “Synapsin I Antibody, 10 µg”

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Synapsin I Antibody, 10 µg
Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

You may also like…