Survival Motor Neuron (SMN) Antibody

Catalog #: 1904-SMN Category: Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G purified tissue culture supernatant
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:4000ICC 1:50-1:500
Species:
Mouse monoclonal
Gene Name:
SMN
Molecular Weight:
~35 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1904-SMN, RRID:AB_2801576
Antigen/Purification: ExpandCollapse

The antigen is recombinant human his-tagged SMN protein expressed in and purified from E. coli. The epitope recognized by the 4B7 clone binds to the region encoded by exon 1.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Survival Motor Neuron (SMN) protein, also known as Gemin1, is derived from the SMN gene which has two nearly identical copies located on chromosome 5q13, SMN1 and SMN2 (Lefebvre et al, 1995). SMA, Spinal Muscular Atrophy, is a neurodegenerative disease caused by mutations of the SMN gene that result in a loss of motor neurons and subsequent progressive limb and trunk muscular atrophy and paralysis (Crawford et al, 1996). SMN1 produces functional, full-length SMN protein, while SMN2 encodes a truncated form of SMN protein that is unstable and defective (Wolstencroft et al., 2005). SMN2 plays a key role in the development of SMA in that the number of SMN2 copies modulates disease severity (Monani et al, 2000). The SMN protein is expressed ubiquitously and found in the cytoplasm as well as nuclear Cajal bodies (Young et al, 2000).

Synonyms: ExpandCollapse
  • BCD541 antibody
  • Component of gems 1 antibody
  • Gemin 1 antibody
  • Gemin-1 antibody
  • OTTHUMP00000125198 antibody
  • OTTHUMP00000223567 antibody
  • OTTHUMP00000223568 antibody
  • OTTHUMP00000224066 antibody
  • OTTHUMP00000226924 antibody
  • SMA 1 antibody
  • SMA 2 antibody
  • SMA 3 antibody
  • SMA 4 antibody
  • SMA antibody
  • [email protected] antibody
  • SMA1 antibody
  • SMA2 antibody
  • SMA3 antibody
  • SMA4 antibody
  • SMN antibody
  • SMN_HUMAN antibody
  • SMN1 antibody
  • SMN2 antibody
  • SMNT antibody
  • Survival motor neuron protein antibody
  • Survival of motor neuron 1, telomeric antibody
  • T-BCD541 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for up to one year after date of receipt. Avoid freeze/thaw cycles.

Product Specific References:

 Coady, T.H., Baughan, T.D., Shababi, M., Passini, M.A. and Lorson, C.L., 2008. Development of a single vector system that enhances trans-splicing of SMN2 transcripts. PLoS One, 3(10), p.e3468.

Wolstencroft, E.C., Mattis, V., Bajer, A.A., Young, P.J. and Lorson, C.L., 2005. A non-sequence-specific requirement for SMN protein activity: the role of aminoglycosides in inducing elevated SMN protein levels. Human molecular genetics, 14(9), pp.1199-1210.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.

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