Affinity purified from pooled serum. Learn more.

SNAP25 (Ser187) Antibody

Catalog #: p1558-187 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Non-human primate, Zebra fish, Guinea Pig
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
SNAP25
Molecular Reference:
~25 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1558-187, RRID:AB_2492239
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on Ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since Ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Synonyms: ExpandCollapse

• bA416N4.2 antibody
• Bdr antibody
• CMS18 antibody
• dJ1068F16.2 antibody
• FLJ23079 antibody
• HGNC:11132 antibody
• MGC105414 antibody
• MGC139754 antibody
• Resistance to inhibitors of cholinesterase 4 homolog antibody
• RIC 4 antibody
• RIC4 antibody
• SEC 9 antibody
• SEC9 antibody
• SNAP 25 antibody
• SNAP antibody
• SNAP-25 antibody
• SNAP-25B antibody
• SNAP25 antibody
• SNP 25 antibody
• SNP25 antibody
• SNP25_HUMAN antibody
• sp antibody
• SUP antibody
• Super protein antibody
• Synaptosomal associated 25 kDa protein antibody
• Synaptosomal associated protein antibody
• Synaptosomal associated protein 25 antibody
• Synaptosomal associated protein 25kDa antibody
• Synaptosomal-associated 25 kDa protein antibody
• Synaptosomal-associated protein 25 antibody
• Synaptosomal-associated protein, 25-KD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Ando K, Kudo Y, Takahashi M (2005) Negative regulation of neurotransmitter release by calpain: a possible specific involvement of Snap25 cleavage. J Neurochem 94(3):651-8.

Fatemi SH, Earle JA, Stary JM, Lee S, Sedgwick J (2001) Altered levels of the synaptosomal associated protein Snap25 in hippocampus of patients with mood disorders and Schizophrenia. Neuroreport. 12(15): 3257-62.

Fatemi SH, Sidwell R, Kist D, Akhter P, Meltzer HY, Bailey K, Thuras P, Sedgwick J (1998) Differential expression of synaptosome-associated protein 25 kDa (Snap25) in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Res 800(1):1-9.

Greber S, Cairns N, Lubec G, Fountoulakis M (1999) Decreased levels of the synaptosomal associated protein 25 in the brains of patients with Down Syndrome and Alzheimer’s Disease. Electrophoresis 20 (4-5):928-34.

Pozzi D, Condliffe S, Bozzi Y, Chikhladze M, Grumelli C, Proux-Gillardeaux V, Takahashi M, Franceschetti S, Verderio C, Matteoli M (2008) Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. PNAS 105: 323-328.

Shu Y, Liu X, Yang Y, Takahashi M, Gillis KD (2008) Phosphorylation of SNAP-25 at Ser187 Mediates Enhancement of Exocytosis by a Phorbol Ester in INS-1 Cells. J of Neurosci 28: 21-30.

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SNAP25 Ser187 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 400 units/100ul lysate for 30 minutes, RT).

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