Affinity purified from pooled serum. Learn more.

SNAP25 (Ser187) Antibody

We are the original manufacturer of our SNAP25 (Ser187) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. It works great in WB.

Catalog #: p1558-187 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Guinea Pig, Human, Non-human primate, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
SNAP25
Molecular Weight:
~25 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1558-187, RRID:AB_2492239
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on Ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since Ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Synonyms: ExpandCollapse

• bA416N4.2 antibody
• Bdr antibody
• CMS18 antibody
• dJ1068F16.2 antibody
• FLJ23079 antibody
• HGNC:11132 antibody
• MGC105414 antibody
• MGC139754 antibody
• Resistance to inhibitors of cholinesterase 4 homolog antibody
• RIC 4 antibody
• RIC4 antibody
• SEC 9 antibody
• SEC9 antibody
• SNAP 25 antibody
• SNAP antibody
• SNAP-25 antibody
• SNAP-25B antibody
• SNAP25 antibody
• SNP 25 antibody
• SNP25 antibody
• SNP25_HUMAN antibody
• sp antibody
• SUP antibody
• Super protein antibody
• Synaptosomal associated 25 kDa protein antibody
• Synaptosomal associated protein antibody
• Synaptosomal associated protein 25 antibody
• Synaptosomal associated protein 25kDa antibody
• Synaptosomal-associated 25 kDa protein antibody
• Synaptosomal-associated protein 25 antibody
• Synaptosomal-associated protein, 25-KD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fatemi, S.H., Earle, J.A., Stary, J.M., Lee, S. and Sedgewick, J., 2001. Altered levels of the synaptosomal associated protein SNAP-25 in hippocampus of subjects with mood disorders and schizophrenia. Neuroreport, 12(15), pp.3257-3262. PMID: 11711867

Fatemi, S.H., Sidwell, R., Kist, D., Akhter, P., Meltzer, H.Y., Bailey, K., Thuras, P. and Sedgwick, J., 1998. Differential expression of synaptosome-associated protein 25 kDa [SNAP-25] in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Research, 800(1), pp.1-9. PMID: 9685568

Greber, S., Lubec, G., Cairns, N. and Fountoulakis, M., 1999. Decreased levels of synaptosomal associated protein 25 in the brain of patients with Down syndrome and Alzheimer’s disease. ELECTROPHORESIS: An International Journal, 20(4‐5), pp.928-934. PMID: 10344268

Ando, K., Kudo, Y. and Takahashi, M., 2005. Negative regulation of neurotransmitter release by calpain: a possible involvement of specific SNAP‐25 cleavage. Journal of Neurochemistry, 94(3), pp.651-658. PMID: 15992386

Pozzi, D., Condliffe, S., Bozzi, Y., Chikhladze, M., Grumelli, C., Proux-Gillardeaux, V., Takahashi, M., Franceschetti, S., Verderio, C. and Matteoli, M., 2008. Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. Proceedings of the National Academy of Sciences, 105(1), pp.323-328. PMID: 18162553

Shu, Y., Liu, X., Yang, Y., Takahashi, M. and Gillis, K.D., 2008. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. Journal of Neuroscience, 28(1), pp.21-30. PMID: 18171919

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…