Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Xenopus, Bovine, Non-human primate, Sheep
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~ 76 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1700-276, RRID:AB_2492237
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr276 of rat SERT.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at thr276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at thr276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the thr276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).