SAP49 Antibody

Catalog #: 1900-SAP Categories: , Datasheet:


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Excellent nuclear marker!

Protein G Purified
Species Tested:
Human, Mouse, Rat
WB 1:2000IHC 1:1000ICC 1:1000
Mouse Monoclonal
Gene Name:
Molecular Weight:
~49 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1900-SAP, RRID:AB_2492236
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human SAP49 expressed in and purified from E. Coli.

The antibody is Protein G purified from tissue culture supernatant.

Biological Significance: ExpandCollapse

The spliceosome associated protein of ~49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006).

Synonyms: ExpandCollapse

• AFD1 antibody
• Hsh49 antibody
• MGC10828 antibody
• Pre mRNA splicing factor SF3b 49 kDa subunit antibody
• Pre-mRNA-splicing factor SF3b 49 kDa subunit antibody
• SAP 49 antibody
• SAP49 antibody
• Sf3b4 antibody
• SF3B4 antibody
• SF3B4_HUMAN antibody
• SF3b49 antibody
• SF3b50 antibody
• Spliceosomal protein antibody
• Spliceosome associated protein (U2 snRNP) antibody
• Spliceosome associated protein 49 antibody
• Spliceosome-associated protein 49 antibody
• Splicing factor 3b subunit 4 49kDa antibody
• Splicing factor 3B subunit 4 antibody


100 µl in PBS + 50% glycerol + 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols


HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Champion-Arnaud P, Reed R. (1994) The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev. 8(16):1974-83. PMID: 7958871

Terada Y, Yasuda Y. (2006) Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145. Mol Cell Biol. 26(21):8149-58. PMID: 16923959

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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SAP49 Antibody
Western blot of HeLa lysate showing specific immunolabeling of the ~49 kDa SAP49 protein.

Click here to view our Western blotting and lysate preparation protocols.

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