Affinity purified from pooled serum. Learn more.

S6 Kinase 1 (Thr449) Antibody

Catalog #: p1705-449 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Arabidopsis
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
S6K1
Molecular Weight:
~53 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1705-449, RRID:AB_2492235
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr449 of Arabidopsis S6K1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).

Synonyms: ExpandCollapse

• 70 kDa ribosomal protein S6 kinase 1 antibody
• KS6B1_HUMAN antibody
• p70 alpha antibody
• P70 beta 1 antibody
• p70 ribosomal S6 kinase alpha antibody
• p70 ribosomal S6 kinase beta 1 antibody
• p70 S6 kinase alpha antibody
• P70 S6 Kinase antibody
• p70 S6 kinase, alpha 1 antibody
• p70 S6 kinase, alpha 2 antibody
• p70 S6K antibody
• p70 S6K-alpha antibody
• p70 S6KA antibody
• p70(S6K) alpha antibody
• p70(S6K)-alpha antibody
• p70-alpha antibody
• p70-S6K 1 antibody
• p70-S6K antibody
• P70S6K antibody
• P70S6K1 antibody
• p70S6Kb antibody
• PS6K antibody
• Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
• Ribosomal protein S6 kinase beta 1 antibody
• Ribosomal protein S6 kinase beta-1 antibody
• Ribosomal protein S6 kinase I antibody
• RPS6KB1 antibody
• S6K antibody
• S6K-beta-1 antibody
• S6K1 antibody
• Serine/threonine kinase 14 alpha antibody
• Serine/threonine-protein kinase 14A antibody
• STK14A antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Dufner A, Thomas G (1999) Ribosomal S6 kinase signalin g and the control of translation. Exp Cell Res 253(1):100-9.

Mahfouz MM, Kim S, Delauney AJ, Verma DP (2006) Arabidopsis TARGET OF RAPAMYCIN interacts with RAPTOR, which regulates the activity of S6 Kinase in response to osmotic stress signals. The Plant Cell 18:477-490.

Pearson RB, Dennis PB, Han JW, Williamson NA, Kozma SC, Wettenhall RE, Thomas G (1995) The principal target of rapamycin-induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain. EMBO J. 14(21):5 279-87.

Schepetilnikov M, Kobayashi K, Geldreich A, Caranta C, Robaglia C, Keller M, Ryabova L (2011) Viral factor TAV recruits TOR/S6K1 signalling to activate reinitiation after long ORF translation. EMBO J. 30:1343-13556.

Schepetilnikov M, Dimitrova M, Mancera-Martinez E, Geldreich A, Keller M, Ryabova L (2013) TOR and S6K1 promote translation reinitiation of uORF-containing mRNAs via phosphorylation of eIF3h. EMBO J, 32:1087-1102.

Xiong Y, Sheen J (2012) Rapamycin and glucose-Target of Rapamycin (TOR) protein signaling in plants. J Biol Chem 287(4):2836-2842.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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S6 Kinase 1 Thr449 Antibody
Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).

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