Rhodopsin Antibody

Catalog #: 1840-RHO Categories: , , Datasheet:

$365.00

Labels rhodopsin localized in rod membranes of ROS.

Size:
100 µl
Formulation:
Protein G Purified
Specificity:
Most mammals
Applications:
WB 1:1,000IHC 1:100
Species:
Mouse monoclonal
Gene Name:
RHO
Molecular Reference:
~39 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1840-RHO, RRID:AB_2492232
Antigen/Purification: ExpandCollapse

The antigen is a bovine rhodopsin.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific References

Kim, J.Y., Park, R., Lee, J.H.J., Shin, J., Nickas, J., Kim, S. and Cho, S.H., 2016. Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Developmental Biology.  PMID: 27616714
Cho, S. H., Song, J. Y., Shin, J., & Kim, S. (2016). Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC ophthalmology, 16(1), 193. PMID: 27809828

 

Rhodopsin Antibody
Immunostaining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

You may also like…