Rhodopsin Antibody

Catalog #: 1840-RHO Categories: , , , Datasheet:

$119.00$380.00

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Labels rhodopsin localized in rod membranes of ROS.

Formulation:
Protein G Purified
Species Tested:
Amphibians and Most mammals
Applications:
WB 1:4000IHC 1:100-1:200
Species:
Mouse monoclonal
Gene Name:
RHO
Molecular Weight:
~39 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1840-RHO, RRID:AB_2492232
Antigen/Purification: ExpandCollapse

The antigen is a bovine rhodopsin.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.

Synonyms: ExpandCollapse

• CSNBAD1 antibody
• MGC138309 antibody
• MGC138311 antibody
• OPN 2 antibody
• OPN2 antibody
• opsd antibody
• OPSD_HUMAN antibody
• opsin 2 antibody
• Opsin 2 rod pigment antibody
• Opsin-2 antibody
• Opsin2 antibody
• Retinitis Pigmentosa 4 antibody
• Retinitis pigmentosa 4 autosomal dominant antibody
• RHO antibody
• Rhodopsin antibody
• RP 4 antibody
• RP4 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Mouse retinal section

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse


Immunohistochemistry: Mouse
PMID Dilution Publication
31254513 1:300 Argyriou, C., et al. 2019. A longitudinal study of retinopathy in the PEX1-Gly844Asp mouse model for mild Zellweger Spectrum Disorder. Experimental Eye Research, p.107713.
31145883 1:200 Cho, S.H., et al. 2019. Targeted deletion of Crb1/Crb2 in the optic vesicle models key features of leber congenital amaurosis 8. Developmental biology, May 28. pii: S0012-1606(19)30027-2.
27809820 not listed Cho, S.H., et al. 2016. Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC ophthalmology, 16(1), 193.
27616714 1:200 Kim, J.Y., et al. 2016. Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Developmental Biology. Nov 15;419(2):336-347.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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