Retinoic Acid Receptor Antibody, β-Isotype, Human

Our Retinoic Acid Receptor beta-isotype mouse monoclonal antibody is Protein G purified. It is excellent for use in WB.

Catalog #: 1340-RXRB Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Protein G Purified
Species Tested:
Human, Rat
Expected Reactivity:
Canine, Guinea Pig, Mouse, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
RARB
Molecular Weight:
~48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1340-RXRB, RRID:AB_2492229
Antigen/Purification: ExpandCollapse

The antigen is a  peptide corresponding to amino acids from N-terminal region of human retinoic acid receptor, β-isotype. The antigen is identical in both β1 and β2 isoforms.

The antibody is prepared from tissue culture supernatant by ammonium sulfate precipitation followed by purification on a Protein G column.

Biological Significance: ExpandCollapse

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The Retinoid X receptors (RXRs) family (RXRα, β and γ) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors (RARs). RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).

Synonyms: ExpandCollapse

• HAP antibody
• HBV-activated protein antibody
• NR1B2 antibody
• Nuclear receptor subfamily 1 group B member 2 antibody
• RAR B antibody
• RAR beta antibody
• RAR epsilon antibody
• RAR-beta antibody
• RAR-epsilon antibody
• RARB antibody
• RARB_HUMAN antibody
• Retinoic acid receptor beta 2 antibody
• Retinoic acid receptor beta 4 antibody
• Retinoic acid receptor beta 5 antibody
• Retinoic acid receptor beta antibody
• Retinoic acid receptor beta polypeptide antibody
• RRB2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Abu, J., Batuwangala, M., Herbert, K. and Symonds, P., 2005. Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. The Lancet Oncology, 6(9), pp.712-720. PMID: 16129372

Joshi, S., Guleria, R., Pan, J., DiPette, D. and Singh, U.S., 2006. Retinoic acid receptors and tissue-transglutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene, 25(2), p.240. PMID: 16158052

Lane, M.A. and Bailey, S.J., 2005. Role of retinoid signalling in the adult brain. Progress in Neurobiology, 75(4), pp.275-293. PMID: 15882777

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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