Retinoic Acid Receptor Antibody, β-Isotype, Human

Catalog #: 1340-RXRB Category: Datasheet:

$365.00

Mouse monoclonal antibody

Size:
100 µl
Formulation:
Protein G Purified
Specificity:
Rat, Mouse, Human, Non-human primate, Canine, Guinea Pig
Applications:
WB 1:1,000
Species:
Mouse
Molecular Reference:
~48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1340-RXRB, RRID:AB_2492229
Antigen/Purification: ExpandCollapse

The antigen is a  peptide corresponding to amino acids from N-terminal region of human retinoic acid receptor, β-isotype. The antigen is identical in both β1 and β2 isoforms.

The antibody is prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a Protein G column.

Biological Significance: ExpandCollapse

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The Retinoid X receptors (RXRs) family (RXRα, β and γ) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors (RARs). RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720.

Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027.

Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293.

Retinoic Acid Receptor Antibody, β-Isotype, Human
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~48 kDa RAR-β isotype.

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