Affinity purified from pooled serum. Learn more.

REDD1 (Thr23/25) Antibody

Catalog #: p1610-2325 Category: Datasheet:


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Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Human, Non-human primate
WB 1:500
Rabbit Polyclonal
Molecular Reference:
~34 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1610-2325, RRID:AB_2492227
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr25, Thr23 and Ser19 through the activity of GSK3b (Katiyar et al, 2009).


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Schwarzer r, Tondera D, Arnold W, Giese K, Klippel A, Kaufmann J (2005) REDD1 integrates hypoxia-mediated survival signaling downstream of phosphatidylinositol 3-kinase. Oncogene 24(7): 1138-49.

Katiyar S, Liu E, Knutzen CA, Lang ES, Lombardo CR, Sankar S, Toth JI, Petroski MD, Ronai Z, Chiang GC (2009) REDD1, an inhibitor of mTOR signaling, is regulated by the CUL4A-DDB1 ubiquitin ligase. EMBO Rep 10(8):866-72.

REDD1 Thr23/25 Antibody
Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

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