Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Human, Non-human primate
- WB 1:500
- Rabbit Polyclonal
- Molecular Reference:
- ~34 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1610-2325, RRID:AB_2492227
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr25, Thr23 and Ser19 through the activity of GSK3b (Katiyar et al, 2009).