RBPMS Antibody, Rabbit

Catalog #: 1830-RBPMS Categories: , , , , Datasheet:

$109.00$365.00

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Retinal ganglion cell marker for IHC and ICC!

Formulation:
Affinity purified
Species Tested:
Human, Mouse, Non-human primate, Rat, Tree Shrew, Zebrafish
Species Reactivity:
Rabbit, Guinea Pig
Applications:
WB 1:1000IHC 1:100-1:2000ICC 1:200-1:300Flow 1:400
Species:
Rabbit Polyclonal
Gene Name:
RBPMS
Molecular Reference:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1830-RBPMS, RRID:AB_2492225
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.

The antibody is prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the peptide used as antigen.

Biological Significance: ExpandCollapse

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014)

Synonyms: ExpandCollapse

• FLJ32971 antibody
• Heart and RRM expressed sequence antibody
• Hermes antibody
• RBP MS antibody
• RBP-MS antibody
• Rbpms antibody
• RBPMS_HUMAN antibody
• RNA binding protein gene with multiple splicing antibody
• RNA-binding protein with multiple splicing antibody

Storage

100 µl in PBS + 0.03% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocol

Immunohistochemistry- Mouse RCG’s

Product specific protocol using mouse RCG’s harvested from adult mice.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Flow Cytometry: Human Stem Cells

Immunocytochemistry: Human Stem Cells | Mouse RCG’s | Rat RCG’s

Immunohistochemistry: Mouse | Primate | Rat | Tree Shrew| Zebrafish


Flow Cytometry: Human Stem Cells
PMID Dilution Publication
27826002 1:400 Hunt, N.C., et al. 2017. 3D culture of human pluripotent stem cells in RGD-alginate hydrogel improves retinal tissue development. Acta Biomaterialia. 2017 Feb;49:329-343.


Immunocytochemistry: Human Stem Cells
PMID Dilution Publication
27826002 1:200 Hunt, N.C., et al. 2017. 3D culture of human pluripotent stem cells in RGD-alginate hydrogel improves retinal tissue development. Acta Biomaterialia. 2017 Feb;49:329-343.


Immunocytochemistry: Mouse RCG’s
PMID Dilution Publication
30796289 1:250 Park, Y.H., et al. 2019. Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice. Scientific reports, 9(1), p.2594.


Immunocytochemistry: Rat RCG’s
PMID Dilution Publication
27826002 1:300 Galvao, J., et al. 2018. The Krüppel-Like Factor Gene Target Dusp14 Regulates Axon Growth and Regeneration. Investigative ophthalmology & visual science, 59(7), pp.2736-2747.


Immunohistochemistry: Mouse
PMID Dilution Publication
30831136 1:200 Hikida, T., et al. 2019. Adolescent psychosocial stress enhances sensitization to cocaine exposure in genetically vulnerable mice. Neuroscience research, pii: S0168-0102(19)30021-5.
30392793 not listed Tiriac, A., et al. 2018. Light Prior to Eye Opening Promotes Retinal Waves and Eye-Specific Segregation. Neuron. 100(5):1059-1065.
30062914 1ug/ul Simpson, E., et al. 2018. New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Human gene therapy, (ja). Oct 2.
29950598 1:200 Mdzomba, J.B., et al. 2018. Nogo-A inactivation improves visual plasticity and recovery after retinal injury. Cell death & disease, 9(7), p.727.
29931057 1:1000 Agostinone, J., et al. 2018. Insulin signalling promotes dendrite and synapse regeneration and restores circuit function after axonal injury. Brain. Jul 1;141(7):1963-1980.
29434244 1:250 Shen, G., et al. 2018. Characterization of Retinal Ganglion Cell and Optic Nerve Phenotypes Caused by Sustained Intracranial Pressure Elevation in Mice. Scientific reports, 8(1), p.2856.
29241535 1:2000 Seabrook, T.A., et al. 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell reports, 21(11), pp.3049-3064.
29106385 not listed Livne-Bar, I., et al. 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation, 127(12).
29067402 1:500 Liang, C., et al. 2017. Optic nerve hypoplasia is a pervasive subcortical pathology of visual system in neonates. Investigative ophthalmology & visual science, 58(12), pp.5485-5496.
28871032 1:300 Apara, A., et al. 2017. KLF9 and JNK3 Interact to Suppress Axon Regeneration in the Adult CNS. Journal of Neuroscience, pp.0643-16.
28774322 1:1000 Chiasseu, M., et al. 2017. Tau accumulation in the retina promotes early neuronal dysfunction and precedes brain pathology in a mouse model of Alzheimer’s disease. Molecular neurodegeneration, 12(1), p.58.
28607486 1:500 Liddelow, S.A., et al. 2017. Neurotoxic reactive astrocytes are induced by activated microglia. Nature, 541(7638), p.481.
28607399 1:500 Assali, A., et al. 2017. RIM1/2 are required in retinal ganglion cells for the refinement of ipsilateral axons and eye-specific segregation. Scientific Reports. Jun 12;7(1):3236.
28262325 1:200 Joly, S., et al. 2017. Non-amyloidogenic processing of amyloid beta precursor protein is associated with retinal function improvement in aging male APP swe/PS1?E9 mice. Neurobiology of Aging. 2017 May;53:181-191.
28099414 1:500 Liddelow, S.A., et al. 2017. Neurotoxic reactive astrocytes are induced by activated microglia. Nature, 541(7638), p.481.
27699209 not listed Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of familial dysautonomia. eNeuro, Sep 27;3(5).
27685630 not listed Livne-Bar, I., et al. 2016. Pharmacologic inhibition of reactive gliosis blocks TNF-?-mediated neuronal apoptosis. Cell death & disease, Sep 29;7(9):e2386.
27654429 1:400 Chen, L. et al. 2016. Heterozygous Pitx2 Null Mice Accurately Recapitulate the Ocular Features of Axenfeld-Rieger Syndrome and Congenital Glaucoma. Investigative Ophthalmology & Visual Science, 57(11), pp.5023-5030.
27399843 1:1000 Lim, J. H. A., et al. 2016. Neural activity promotes long-distance, target-specific regeneration of adult retinal axons. Nature Neuroscience. 2016 Aug;19(8):1073-84.


Immunohistochemistry: Primate
PMID Dilution Publication
29954626 1:100 Sauter, M.M., et al 2018. Toll-like receptors 4, 5, 6 and 7 are constitutively expressed in non-human primate retinal neurons. Journal of Neuroimmunology. Sep 15;322:26-35.


Immunohistochemistry: Rat
PMID Dilution Publication
30837658 1:250 van der Merwe, Y., et al. 2019. Matrix-bound nanovesicles prevent ischemia-induced retinal ganglion cell axon degeneration and death and preserve visual function. Scientific Reports, 9(1):3482.
29860460 1:300 Galvao, J., et al. 2018. The Krüppel-Like Factor Gene Target Dusp14 Regulates Axon Growth and Regeneration. Investigative ophthalmology & visual science, 59(7), pp.2736-2747.
27163643 1ug/ml Vargas, J. L. C., et al. 2016. The glial cell modulator ibudilast attenuates neuroinflammation and enhances retinal ganglion cell viability in glaucoma through protein kinase A signaling. Neurobiology of disease, 93, 156-171.
26539914 1:500 Shabanzadeh, A. P., et al. 2015. Targeting caspase-6 and caspase-8 to promote neuronal survival following ischemic stroke. Cell death & disease, 6(11), e1967.
26338321 0.1ug/ml Vargas, J. L. C., et al. 2015. Soluble Tumor Necrosis Factor Alpha Promotes Retinal Ganglion Cell Death in Glaucoma via Calcium-Permeable AMPA Receptor Activation. The Journal of Neuroscience, 35(35), 12088-12102.
25450063 1:300 Reifler, A. N., et al. 2015. The rat retina has five types of ganglion-cell photoreceptors. Experimental eye research, 130, 17-28.


Immunohistochemistry: Tree Shrew
PMID Dilution Publication
29238991 1:500 Johnson, E.N., et al. 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology.


Immunohistochemistry: Zebrafish
PMID Dilution Publication
30382466 1:100 Geeraerts, E., et al. 2018. Extensive growth is followed by neurodegenerative pathology in the continuously expanding adult zebrafish retina. Biogerontology, 20(1):109-125.
29378863 1:300 Elsaeidi, F., et al. 2018. Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice. J Neurosci, 38(9):2246-2261.

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RBPMS Antibody, Rabbit
Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunostaining of mouse retinal ganglion cells showing specific immunolabeling of RBPMS in red. Photo courtesy of Allen Rodriguez, University of California, Los Angeles.
Download the protocol used for this image:

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