RBPMS Antibody, Rabbit

$365.00

Retinal ganglion cell marker for IHC!

Size:
100 µl
Formulation:
Affinity purified
Specificity:
Rat, Mouse, Human, Non-human primate, Rabbit, Guinea Pig
Applications:
WB 1:1,000IHC 1: 500
Species:
Rabbit polyclonal
Gene Name:
RBPMS
Molecular Reference:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1830-RBPMS, RRID:AB_2492225
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.

The antibody is prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the peptide used as antigen.

Biological Significance: ExpandCollapse

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014)

Storage

100 µl in PBS + 0.03% sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific References

Mdzomba, J.B., Jordi, N., Rodriguez, L., Joly, S., Bretzner, F. and Pernet, V., 2018. Nogo-A inactivation improves visual plasticity and recovery after retinal injury. Cell death & disease, 9(7), p.727. PMID: 29950598

Galvao, J., Iwao, K., Apara, A., Wang, Y., Ashouri, M., Shah, T.N., Blackmore, M., Kunzevitzky, N.J., Moore, D.L. and Goldberg, J.L., 2018. The Krüppel-Like Factor Gene Target Dusp14 Regulates Axon Growth and Regeneration. Investigative ophthalmology & visual science, 59(7), pp.2736-2747. PMID: 29860460

Shen, G., Link, S., Kumar, S., Nusbaum, D.M., Dennis, Y.T., Fu, Y., Wu, S.M. and Frankfort, B.J., 2018. Characterization of Retinal Ganglion Cell and Optic Nerve Phenotypes Caused by Sustained Intracranial Pressure Elevation in Mice. Scientific reports, 8(1), p.2856. PMID: 29434244

Elsaeidi, F., Macpherson, P., Mills, E.A., Jui, J., Flannery, J.G. and Goldman, D., 2018. Notch suppression collaborates with Ascl1 and Lin28 to unleash a regenerative response in fish retina, but not in mice. Journal of Neuroscience, pp.2126-17. PMID: 29378863

Carvalho, L.S., Xiao, R., Wassmer, S., Langsdorf, A., Zinn, E., Pacouret, S., Shah, S., Comander, J.I., Kim, L., Lim, L. and Vandenberghe, L.H., 2018. Synthetic adeno-associated viral vector efficiently targets mouse and non-human primate retina in vivo. Human gene therapy, (ja). PMID: 29325457

Johnson, E.N., Westbrook, T., Shayesteh, R., Chen, E.L., Schumacher, J.W., Fitzpatrick, D. and Field, G.D., 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology. PMID: 29238991

Seabrook, T.A., Dhande, O.S., Ishiko, N., Wooley, V.P., Nguyen, P.L. and Huberman, A.D., 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell reports, 21(11), pp.3049-3064. PMID: 29241535

Livne-Bar, I., Wei, J., Liu, H.H., Alqawlaq, S., Won, G.J., Tuccitto, A., Gronert, K., Flanagan, J.G. and Sivak, J.M., 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation, 127(12). PMID: 29106385

Apara, A., Galvao, J., Wang, Y., Blackmore, M., Trillo, A., Iwao, K., Brown, D.P., Fernandes, K.A., Huang, A., Nguyen, T. and Ashouri, M., 2017. KLF9 and JNK3 Interact to Suppress Axon Regeneration in the Adult CNS. Journal of Neuroscience, pp.0643-16. PMID: 28871032

Chiasseu, M., Alarcon-Martinez, L., Belforte, N., Quintero, H., Dotigny, F., Destroismaisons, L., Velde, C.V., Panayi, F., Louis, C. and Di Polo, A., 2017. Tau accumulation in the retina promotes early neuronal dysfunction and precedes brain pathology in a mouse model of Alzheimer’s disease. Molecular Neurodegeneration, 12(1), p.58. PMID: 28774322

Assali, A., Le Magueresse, C., Bennis, M., Nicol, X., Gaspar, P. and Rebsam, A., RIM1/2 are required in retinal ganglion cells for the refinement of ipsilateral axons and eye-specific segregation. Scientific Reports. 2017, Jun 12;7(1):3236.  PMID: 28607399

Joly, S., Lamoureux, S. and Pernet, V., 2017. Non-amyloidogenic processing of amyloid beta precursor protein is associated with retinal function improvement in aging male APP swe/PS1ΔE9 mice. Neurobiology of Aging. 2017 May;53:181-191. PMID: 28262325

Liddelow, S.A., Guttenplan, K.A., Clarke, L.E., Bennett, F.C., Bohlen, C.J., Schirmer, L., Bennett, M.L., Münch, A.E., Chung, W.S., Peterson, T.C. and Wilton, D.K., 2017. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017 Jan 26;541(7638):481-487. PMID: 28099414

Hunt, N.C., Hallam, D., Karimi, A., Mellough, C.B., Chen, J., Steel, D.H. and Lako, M., 2016. 3D culture of human pluripotent stem cells in RGD-alginate hydrogel improves retinal tissue development. Acta Biomaterialia.  2017 Feb;49:329-343.  PMID: 27826002

Chen, L. and Gage, P.J., 2016. Heterozygous Pitx2 Null Mice Accurately Recapitulate the Ocular Features of Axenfeld-Rieger Syndrome and Congenital Glaucoma. Investigative Ophthalmology & Visual Science, 57(11), pp.5023-5030. PMID: 27654429

Lim, J. H. A., Stafford, B. K., Nguyen, P. L., Lien, B. V., Wang, C., Zukor, K., He, Z. & Huberman, A. D. (2016). Neural activity promotes long-distance, target-specific regeneration of adult retinal axons. Nature Neuroscience. 2016 Aug;19(8):1073-84. PMID: 27399843

Vargas, J. L. C., Belforte, N., & Di Polo, A. (2016). The glial cell modulator ibudilast attenuates neuroinflammation and enhances retinal ganglion cell viability in glaucoma through protein kinase A signaling. Neurobiology of disease, 93, 156-171. PMID: 27163643

Shabanzadeh, A. P., D’onofrio, P. M., Monnier, P. P., & Koeberle, P. D. (2015). Targeting caspase-6 and caspase-8 to promote neuronal survival following ischemic stroke. Cell death & disease, 6(11), e1967. PMID: 26539914

Vargas, J. L. C., Osswald, I. K., Unsain, N., Aurousseau, M. R., Barker, P. A., Bowie, D., & Di Polo, A. (2015). Soluble Tumor Necrosis Factor Alpha Promotes Retinal Ganglion Cell Death in Glaucoma via Calcium-Permeable AMPA Receptor Activation. The Journal of Neuroscience, 35(35), 12088-12102. PMID: 26338321

Reifler, A. N., Chervenak, A. P., Dolikian, M. E., Benenati, B. A., Meyers, B. S., Demertzis, Z. D., Lynch, A.M., Li, B.Y., Wachter, R.D., Abufarha, F.S., Dulka, E.A., Pack, W., Zhao, X., & Wong, K. Y. (2015). The rat retina has five types of ganglion-cell photoreceptors. Experimental eye research, 130, 17-28. PMID: 25450063

RBPMS Antibody, Rabbit
Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
Immunostaining of mouse retinal ganglion cells showing specific immunolabeling of RBPMS in red. Photo courtesy of Allen Rodriguez, University of California, Los Angeles.

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