RBPMS Antibody, Guinea Pig

Catalog #: 1832-RBPMS Categories: , , , , Datasheet:

$109.00$365.00

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Guinea pig antibody – perfect for IHC and ICC staining!

Formulation:
Affinity purified
Species Tested:
Blind Mole Rat, Guinea Pig, Human, Mouse, Non-human primate, Rabbit, Rat, Tree Shrew
Applications:
WB 1:1000IHC 1:50-1:20,000ICC 1:1000
Species:
Guinea Pig Polyclonal
Gene Name:
RBPMS
Molecular Reference:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1832-RBPMS, RRID:AB_2492226
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.

The antibody is prepared from guinea pig serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

Synonyms: ExpandCollapse

• FLJ32971 antibody
• Heart and RRM expressed sequence antibody
• Hermes antibody
• RBP MS antibody
• RBP-MS antibody
• Rbpms antibody
• RBPMS_HUMAN antibody
• RNA binding protein gene with multiple splicing antibody
• RNA-binding protein with multiple splicing antibody

Storage

100 µl in PBS + 0.03% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocol

Immunohistochemistry

Rat Retina.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse Retinal Culture

Immunohistochemistry: Human | Mouse | Primate | Rabbit | Rat | Tree Shrew


Immunocytochemistry: mouse retinal culture
PMID Dilution Publication
30018341 not listed Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature communications, 9(1), p.2759.
29576390 1:1000 Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126.


Immunohistochemistry: human
PMID Dilution Publication
29665009 1:500 Christiansen, A.T., et al. 2018. Localization, distribution, and connectivity of neuropeptide Y in the human and porcine retinas—A comparative study. J Comp Neurol., 526(12):1877-1895.
28399269 not listed Obara, E.A., et al. 2017. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Patients With Diabetic Retinopathy. Investigative Ophthalmology & Visual Science, 58(4), pp.2187-2192.
27583827 1:500 Obara, E. A., et al. 2016. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Severely Staged Glaucoma Patients. Investigative Ophthalmology & Visual Science, 57(11), 4661-4667.


Immunohistochemistry: mouse
PMID Dilution Publication
30640971 1:1000 Blandford, S.N., et al. 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative ophthalmology & visual science, 60(1), pp.183-191.
27391320 1:500 Ghinia, M.G., et al. 2019. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. Journal of Comparative Neurology, 527(1): 187-211.
30487225 1:300 Milosavljevic, N., et al. 2018. Photoreceptive retinal ganglion cells control the information rate of the optic nerve. Proceedings of the National Academy of Sciences, 115(50), pp.E11817-E11826.
30312782 1:2000 Yee, C.W., et al. 2018. Atypical expression and activation of GluN2A and GluN2B-containing NMDA receptors at ganglion cells during retinal degeneration. Neuroscience. 10 Oct.
30197236 1:1000 Duan, X., et al. 2018. Cadherin Combinations Recruit Dendrites of Distinct Retinal Neurons to a Shared Interneuronal Scaffold. Neuron, 99(6), pp.1145-1154.
30076594 1:500 Lilley, B.N., et al. 2018. Genetic access to neurons in the accessory optic system reveals a role for Sema6A in midbrain circuitry mediating motion perception. Journal of Comparative Neurology. Aug 3.
30018341 not listed Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature communications, 9(1), p.2759.
29937281 1:200 Drinnenberg, A., et al. 2018. How Diverse Retinal Functions Arise from Feedback at the First Visual Synapse. Neuron, 99(1):117-134.
29931057 1:1000 Agostinone, J., et al. 2018. Insulin signalling promotes dendrite and synapse regeneration and restores circuit function after axonal injury. Brain. Jul 1;141(7):1963-1980.
29576390 1:50 Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126.
29632360 1:500 Liu, J., et al. 2018. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites. Nature neuroscience, May;21(5):659-670.
29439167 1:800 Ing-Esteves, S., et al. 2018. Combinatorial Effects of Alpha-and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance. Journal of Neuroscience, 38(11), pp.2713-2729.
28760865 1:500 Clements, R., et al. 2017. Dystroglycan maintains inner limiting membrane integrity to coordinate retinal development. Journal of Neuroscience, 37(35):8559-8574.
29241535 1:2000 Seabrook, T.A., et al. 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell reports, 21(11), pp.3049-3064.
29106385 not listed Livne-Bar, I., et al. 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation, 127(12).
28866734 1:4000 Ruzafa, N., et al. 2017. The Retina of Osteopontin deficient Mice in Aging. Molecular Neurobiology, pp.1-9.
28781169 1:5000 Peng, Y.R., et al. 2017. Satb1 Regulates Contactin 5 to Pattern Dendrites of a Mammalian Retinal Ganglion Cell. Neuron. Aug 16;95(4):869-883.e6.
28607486 not listed Sabbah, S., et al. 2017. A retinal code for motion along the gravitational and body axes. Nature, 546(7659), pp.492-497.
28472856 1:1000 Pérez de Sevilla Müller, L., et al. 2017. Multiple cell types form the VIP amacrine cell population. Journal of Comparative Neurology.
27699209 not listed Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of Familial Dysautonomia. eneuro, 3(5), pp.ENEURO-0143.
27001178 not listed Boggio, E. M., et al. 2016. Visual impairment in FOXG1-mutated individuals and mice. Neuroscience, 324, 496-508.
25631988 1:20,000 Pérez de Sevilla Müller, L., et al. 2015. Expression and cellular localization of the voltage?gated calcium channel ?2?3 in the rodent retina. Journal of Comparative Neurology, 523(10), 1443-1460.


Immunohistochemistry: primate
PMID Dilution Publication
30377226 1:1000 Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. J Neurosci.,39(1):78-95.
27568514 1:500 Long, Y., et al. 2016. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine-and Amphetamine-Regulated Transcript (CART). Peptides, 84, 22-35.


Immunohistochemistry: rabbit
PMID Dilution Publication
30377226 1:1000 Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. J Neurosci.,39(1):78-95.


Immunohistochemistry: rat
PMID Dilution Publication
27375437 1:500 Esquiva, G., et al. 2016. Non-image forming light detection by melanopsin, rhodopsin, and long-middlewave (L/W) cone opsin in the subterranean blind mole rat, Spalax ehrenbergi: immunohistochemical characterization, distribution, and connectivity. Frontiers in neuroanatomy 10, p.61.


Immunohistochemistry: tree shrew
PMID Dilution Publication
29238991 1:500 Johnson, E.N., et al. 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology.

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RBPMS Antibody, Guinea Pig
Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Click here to view our Western blotting and lysate preparation protocols.
Purified mouse RGCs in culture that have been probed by FISH for Runx1(red), immunostained with an RGC marker RBPMS(green, catalog #1832-RBPMS), and DAPI(blue). (A) An section where two Runx1+ RGCs outlined with dashed box are shown enlarged below (B,C; cell soma outlined with dashed line). Merged image in the upper panel also includes brightfield, which was used to outline cell soma shape in the insets. Granularized signal (arrowheads) in the insets is a property of the single molecule sensitivity FISH kit. Photo courtesy of Bruce Rheaume from University of Connecticut.
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Learn more about these images in this publication from Nature Communications.

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