RBPMS Antibody, Guinea Pig

$109.00$365.00

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Guinea pig antibody – perfect for IHC staining!

Formulation:
Affinity purified
Specificity:
Rat, Mouse, Human, Non-human primate, Rabbit, Guinea Pig
Applications:
WB 1:1,000IHC 1:500
Species:
Guinea Pig Polyclonal
Gene Name:
RBPMS
Molecular Reference:
~24 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1832-RBPMS, RRID:AB_2492226
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.

The antibody is prepared from guinea pig serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

Storage

100 µl in PBS + 0.03% sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific Protocol

Immunohistochemistry- Rat Tissue

Product specific protocol using RBCs from adult albino Sprague Dawley rats.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References For Applications And Species

Immunohistochemistry- human tissue

Christiansen, A.T., Kiilgaard, J.F., Klemp, K., Woldbye, D.P.D. and Hannibal, J., 2018. Localization, distribution, and connectivity of neuropeptide Y in the human and porcine retinas—A comparative study. J Comp Neurol., 526(12):1877-1895. PMID: 29665009

Obara, E.A., Hannibal, J., Heegaard, S. and Fahrenkrug, J., 2017. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Patients With Diabetic Retinopathy. Investigative Ophthalmology & Visual Science58(4), pp.2187-2192. PMID: 28399269

Obara, E. A., Hannibal, J., Heegaard, S., & Fahrenkrug, J. (2016). Loss of Melanopsin-Expressing Retinal Ganglion Cells in Severely Staged Glaucoma Patients. Investigative Ophthalmology & Visual Science57(11), 4661-4667.  PMID: 27583827

Immunohistochemistry- mouse tissue

Ghinia, M.G., Novelli, E., Sajgo, S., Badea, T.C. and Strettoi, E., 2019. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. Journal of Comparative Neurology, 527(1): 187-211. PMID: 27391320

Milosavljevic, N., Storchi, R., Eleftheriou, C.G., Colins, A., Petersen, R.S. and Lucas, R.J., 2018. Photoreceptive retinal ganglion cells control the information rate of the optic nerve. Proceedings of the National Academy of Sciences, 115(50), pp.E11817-E11826. PMID: 30487225 

Yee, C.W., Ivanova, E., Toychiev, A.H., Willis, D.E. and Sagdullaev, B.T., 2018. Atypical expression and activation of GluN2A and GluN2B-containing NMDA receptors at ganglion cells during retinal degeneration. Neuroscience. 10 Oct. PMID: 30312782

Duan, X., Krishnaswamy, A., Laboulaye, M.A., Liu, J., Peng, Y.R., Yamagata, M., Toma, K. and Sanes, J.R., 2018. Cadherin Combinations Recruit Dendrites of Distinct Retinal Neurons to a Shared Interneuronal Scaffold. Neuron99(6), pp.1145-1154. PMID: 30197236

Lilley, B.N., Sabbah, S., Hunyara, J.L., Gribble, K.D., Al‐Khindi, T., Xiong, J., Wu, Z., Berson, D.M. and Kolodkin, A.L., 2018. Genetic access to neurons in the accessory optic system reveals a role for Sema6A in midbrain circuitry mediating motion perception. Journal of Comparative Neurology. Aug 3. PMID: 30076594

Rheaume, B.A., Jereen, A., Bolisetty, M., Sajid, M.S., Yang, Y., Renna, K., Sun, L., Robson, P. and Trakhtenberg, E.F., 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature communications9(1), p.2759. PMID: 30018341

Drinnenberg, A., Franke, F., Morikawa, R.K., Jüttner, J., Hillier, D., Hantz, P., Hierlemann, A., da Silveira, R.A. and Roska, B., 2018. How Diverse Retinal Functions Arise from Feedback at the First Visual Synapse. Neuron, 99(1):117-134. PMID: 29937281

Agostinone, J., Alarcon-Martinez, L., Gamlin, C., Yu, W.Q., Wong, R.O. and Di Polo, A., 2018. Insulin signalling promotes dendrite and synapse regeneration and restores circuit function after axonal injury. Brain. Jul 1;141(7):1963-1980. PMID: 29931057

Sarin, S., Zuniga-Sanchez, E., Kurmangaliyev, Y.Z., Cousins, H., Patel, M., Hernandez, J., Zhang, K.X., Samuel, M.A., Morey, M., Sanes, J.R. and Zipursky, S.L., 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron98(1), pp.109-126. PMID: 29576390

Liu, J., Reggiani, J.D., Laboulaye, M.A., Pandey, S., Chen, B., Rubenstein, J.L., Krishnaswamy, A. and Sanes, J.R., 2018. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites. Nature neuroscience, May;21(5):659-670. PMID: 29632360

Ing-Esteves, S., Kostadinov, D., Marocha, J., Sing, A.D., Joseph, K.S., Laboulaye, M.A., Sanes, J.R. and Lefebvre, J.L., 2018. Combinatorial Effects of Alpha-and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance. Journal of Neuroscience38(11), pp.2713-2729. PMID: 29439167

Clements, R., Turk, R., Campbell, K.P. and Wright, K.M., 2017. Dystroglycan maintains inner limiting membrane integrity to coordinate retinal development. Journal of Neuroscience, 37(35):8559-8574. PMID: 28760865

Seabrook, T.A., Dhande, O.S., Ishiko, N., Wooley, V.P., Nguyen, P.L. and Huberman, A.D., 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell reports21(11), pp.3049-3064. PMID: 29241535

Livne-Bar, I., Wei, J., Liu, H.H., Alqawlaq, S., Won, G.J., Tuccitto, A., Gronert, K., Flanagan, J.G. and Sivak, J.M., 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation127(12). PMID: 29106385

Ruzafa, N., Pereiro, X., Aspichueta, P., Araiz, J. and Vecino, E., 2017. The Retina of Osteopontin deficient Mice in Aging. Molecular Neurobiology, pp.1-9. PMID: 28866734

Peng, Y.R., Tran, N.M., Krishnaswamy, A., Kostadinov, D., Martersteck, E.M. and Sanes, J.R., 2017. Satb1 Regulates Contactin 5 to Pattern Dendrites of a Mammalian Retinal Ganglion Cell. Neuron. Aug 16;95(4):869-883.e6. PMID: 28781169

Sabbah, S., Gemmer, J.A., Bhatia-Lin, A., Manoff, G., Castro, G., Siegel, J.K., Jeffery, N. and Berson, D.M., 2017. A retinal code for motion along the gravitational and body axes. Nature546(7659), pp.492-497. PMID: 28607486

Pérez de Sevilla Müller, L., Solomon, A., Sheets, K., Hapukino, H., Rodriguez, A. and Brecha, N.C., 2017. Multiple cell types form the VIP amacrine cell population. Journal of Comparative Neurology.  PMID: 28472856

Ueki, Y., Ramirez, G., Salcedo, E., Stabio, M.E. and Lefcort, F., 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of Familial Dysautonomia. eneuro3(5), pp.ENEURO-0143. PMID: 27699209

Boggio, E. M., Pancrazi, L., Gennaro, M., Rizzo, C. L., Mari, F., Meloni, I., Ariani, F., Panighini, A., Novelli, E., Biagioni, M., Strettoi, E., Hayek, J., Rufa, A., Pizzorusso, T., Renieri, A., & Strettoi, E. (2016). Visual impairment in FOXG1-mutated individuals and mice. Neuroscience324, 496-508.  PMID: 27001178

Pérez de Sevilla Müller, L., Sargoy, A., Fernández‐Sánchez, L., Rodriguez, A., Liu, J., Cuenca, N., & Brecha, N. (2015). Expression and cellular localization of the voltage‐gated calcium channel α2δ3 in the rodent retina. Journal of Comparative Neurology, 523(10), 1443-1460. PMID: 25631988

Immunohistochemistry- primate tissue

Dhande, O.S., Stafford, B.K., Franke, K., El-Danaf, R., Percival, K.A., Phan, A.H., Li, P., Hansen, B.J., Nguyen, P.L., Berens, P. and Taylor, W.R., 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. J Neurosci.,39(1):78-95. PMID: 30377226

Long, Y., Bordt, A. S., Liu, W. S., Davis, E. P., Lee, S. J., Tseng, L., Chuang, A.Z., Whitaker, C.M., Massey, S.C., Sherman, M.B., & Marshak, D. W. (2016). Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine-and Amphetamine-Regulated Transcript (CART). Peptides84, 22-35. PMID: 27568514

Immunohistochemistry- rat tissue

Esquiva, G., Avivi, A., Hannibal, J., 2016. Non-image forming light detection by melanopsin, rhodopsin, and long-middlewave (L/W) cone opsin in the subterranean blind mole rat, Spalax ehrenbergi: immunohistochemical characterization, distribution, and connectivity. Frontiers in neuroanatomy 10, p.61. PMID: 27375437

Immunohistochemistry- tree shrew tissue

Johnson, E.N., Westbrook, T., Shayesteh, R., Chen, E.L., Schumacher, J.W., Fitzpatrick, D. and Field, G.D., 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology. PMID: 29238991

RBPMS Antibody, Guinea Pig
Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS in green. Photo courtesy of Brian Choi, Univ. of Toronto.

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