Affinity purified from pooled serum. Learn more.

Rabphilin 3A (Ser234) Antibody

Rabbit polyclonal antibody

Catalog #: p1553-234 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Mouse
Applications:
WB 1:1000ICC 1:400 Don't see your application?
Host Species:
Rabbit
Gene Name:
RPH3A
Molecular Weight:
~82 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1553-234, RRID:AB_2492222
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of rabphilin 3A.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001).

Synonyms: ExpandCollapse

• Exophilin 1 antibody
• Exophilin-1 antibody
• Exophilin1 antibody
• KIAA0985 antibody
• Rabphilin 3A homolog antibody
• Rabphilin-3A antibody
• Rabphilin3A antibody
• RP3A antibody
• RP3A_HUMAN antibody
• Rph 3a antibody
• Rph3a antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Rat


Western Blot: Rat
PMID Dilution Publication
28823933 not listed Stanic, J., et al. 2017 Rabphilin 3A: A novel target for the treatment of levodopa-induced dyskinesias. Neurobiology of Disease, 108, pp.54-64.
18519737 not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. The Journal of Cell Biology, 181(5), pp.831-846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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