Affinity purified from pooled serum. Learn more.

Rabphilin 3A Antibody

Catalog #: 1825-RBP Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
RPH3A
Molecular Reference:
~82 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1825-RBP, RRID:AB_2492221
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the C-terminal region of rabphilin 3A.

The antibody is  prepared from pooled rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).

Synonyms: ExpandCollapse

• Exophilin 1 antibody
• Exophilin-1 antibody
• Exophilin1 antibody
• KIAA0985 antibody
• Rabphilin 3A homolog antibody
• Rabphilin-3A antibody
• Rabphilin3A antibody
• RP3A antibody
• RP3A_HUMAN antibody
• Rph 3a antibody
• Rph3a antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.

Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMPdependent protein kinases in vitro. J Neurosci 15:2385-2395.

Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond ) 369:493-497.

Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC (1994) Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid- binding protein, depends on rab3A/3C. Neuron 13:885-898.

Smith R, Petersen A, Bates GP, Brundin P, Li JY (2005) Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington’s disease, a possible culprit in synaptic dysfunction. Neurobiol Dis 20:673-684.

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Rabphilin 3A Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A.

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