Affinity purified from pooled serum. Learn more.

Rab3 Antibody

Catalog #: 1805-Rab3 Category: Datasheet:

$365.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Xenopus, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit
Molecular Reference:
~27 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1805-Rab3, RRID:AB_2492220
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of rat Rab 3, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is  prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3’s are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Schluter OM, Schmitz F, Jahn R, Rosenmund C, Sudhof TC (2004) A Complete Genetic Analysis of Neuronal Rab3 Function. J Neuroscience 24(29):6629-6637

Schluter OM, Basu J, Sudhof TC, Rosenmund C (2006) Rab3 Superprimes Synaptic Vesicles for Release: Implications for Short-term Synaptic Plasticity. J Neuroscience 26(4):1239-1246.

Coppola T, Perret-Menoud V, Gattesco S, Magnin S, Pombo I, Blank U, Regazzi R (2002) The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells. J Biochem 362 (Pt.2):273-9.

Rab3 Antibody
Western blot of rat cortical lysate showing specific labeling of the ~27 kDa Rab3 protein.

You may also like…