Affinity purified from pooled serum. Learn more.

Potassium Chloride Cotransporter 2 (KCC2) (Ser940) Antibody

Catalog #: p1551-940 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
KCC2
Molecular Weight:
~135 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1551-940, RRID:AB_2492213
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser940 of rat KCC2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Biological Significance: ExpandCollapse

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).

Synonyms: ExpandCollapse

• Electroneutral potassium chloride cotransporter 2 antibody
• Electroneutral potassium-chloride cotransporter 2 antibody
• Erythroid K Cl cotransporter 2 antibody
• Furosemide sensitive K Cl cotransporter antibody
• hKCC2 antibody
• K-Cl cotransporter 2 antibody
• KCC 2 antibody
• KCC2 antibody
• KIAA1176 antibody
• Neuronal K Cl cotransporter antibody
• Neuronal K-Cl cotransporter antibody
• Potassium Chloride Cotransporter antibody
• Potassium chloride transporter 5 antibody
• rKCC2 antibody
• S12A5 antibody
• S12A5_HUMAN antibody
• SLC12A5 antibody
• Solute carrier family 12 (potassium chloride transporter) member 5 antibody
• Solute carrier family 12 member 5 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Mekhael, W., Begum, S., Samaddar, S., Hassan, M., Toruno, P., Ahmed, M., Gorin, A., Maisano, M., Ayad, M. and Ahmed, Z., 2019. Repeated anodal trans?spinal direct current stimulation results in long?term reduction of spasticity in mice with spinal cord injury. The Journal of physiology. PMID: 30689208

Conway, L.C., Cardarelli, R.A., Moore, Y.E., Jones, K., McWilliams, L.J., Baker, D.J., Burnham, M.P., Bürli, R.W., Wang, Q., Brandon, N.J. and Moss, S.J., 2017. N-ethylmaleimide increases KCC2 activity by modulating transporter phosphorylation. Journal of Biological Chemistry, Dec 29;292(52):21253-21263. PMID: 29092909

Mahadevan, V., Khademullah, C.S., Dargaei, Z., Chevrier, J., Uvarov, P., Kwan, J., Bagshaw, R.D., Pawson, T., Emili, A., De Koninck, Y. and Anggono, V., 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. Elife, 6, p.e28270. PMID: 29028184

Rinetti-Vargas, G., Phamluong, K., Ron, D. and Bender, K.J., 2017. Periadolescent Maturation of GABAergic Hyperpolarization at the Axon Initial Segment. Cell Reports, 20(1), pp.21-29. PMID: 28683314

Huang, Y.J., Lee, K.H., Murphy, L., Garraway, S.M. and Grau, J.W., 2016. Acute spinal cord injury (SCI) transforms how GABA affects nociceptive sensitization. Experimental Neurology, 285, pp.82-95. PMID: 27639636

López-Álvarez, V. M., Modol, L., Navarro, X., Cobianchi, S., & Neurodegenerativas, E. (2015). Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury. Pain. Jun 18. PMID: 26090759

Silayeva L, Deeb TZ, Hines M, Kelley MR, Munoz MB, Lee HHC, Brandon NJ, Dunlop J, Maguire J, Davies PA, Moss SJ. (2015) KCC2 activity is critical in limiting the onset and severity of status epilepticus. PNAS. Mar 2. pii: 201415126. PMID: 25733865

Mòdol, L., Casas, C., Llidó, A., Navarro, X., Pallarès, M., & Darbra, S. (2014). Neonatal allopregnanolone or finasteride administration modifies hippocampal K+ Cl? co-transporter expression during early development in male rats. The Journal of steroid biochemistry and molecular biology, 143, 343-347. PMID: 24861264

Modol, L., Cobianchi, S., & Navarro, X. (2014). Prevention of NKCC1 phosphorylation avoids downregulation of KCC2 in central sensory pathways and reduces neuropathic pain after peripheral nerve injury. PAIN, 155(8), 1577-1590. PMID: 24813295

Mòdol, L., Mancuso, R., Alé, A., Francos-Quijorna, I., & Navarro, X. (2014). Differential effects on KCC2 expression and spasticity of ALS and traumatic injuries to motoneurons. Frontiers in cellular neuroscience, 8:7. PMID: 24478630

Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43. PMID: 21532577

Sarkar J, Wakefield S, MacKenzie G, Moss SJ and Maguire J. (2011) Neurosteroidogenesis is required for the physiological response to stress: role of neurosteroid-sensitive GABAA receptors. J Neurosci. 31(50):18198-210. PMID: 22171026

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Potassium Chloride Cotransporter 2 (KCC2) ser940 Antibody
Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

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