Affinity purified from pooled serum. Learn more.

Potassium Chloride Cotransporter 2 (KCC2) (Ser940) Antibody

Catalog #: p1551-940 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Canine, Human, Non-human primate
Applications:
WB 1:1000IP 40ul of antibody per 1mg lysate Don't see your application?
Species:
Rabbit
Gene Name:
KCC2
Molecular Weight:
~135 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1551-940, RRID:AB_2492213
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser940 of rat KCC2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Biological Significance: ExpandCollapse

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).

Synonyms: ExpandCollapse

• Electroneutral potassium chloride cotransporter 2 antibody
• Electroneutral potassium-chloride cotransporter 2 antibody
• Erythroid K Cl cotransporter 2 antibody
• Furosemide sensitive K Cl cotransporter antibody
• hKCC2 antibody
• K-Cl cotransporter 2 antibody
• KCC 2 antibody
• KCC2 antibody
• KIAA1176 antibody
• Neuronal K Cl cotransporter antibody
• Neuronal K-Cl cotransporter antibody
• Potassium Chloride Cotransporter antibody
• Potassium chloride transporter 5 antibody
• rKCC2 antibody
• S12A5 antibody
• S12A5_HUMAN antibody
• SLC12A5 antibody
• Solute carrier family 12 (potassium chloride transporter) member 5 antibody
• Solute carrier family 12 member 5 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunoprecippitation: Mouse

Western Blot: Mouse | Rat


Immunoprecipitation: Mouse
PMID Dilution Publication
29028184 40ul of antibody per 1mg lysate Mahadevan, V., et al. 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. Elife, 6, p.e28270.


Western Blot: Mouse
PMID Dilution Publication
31308096 1:1000 Gomez, C.D., et al. 2019. Early life inflammation increases CA1 pyramidal neuron excitability in a sex and age dependent manner through a chloride homeostasis disruption. Journal of Neuroscience, pp.2973-18.
30689208 1:1000 Mekhael, W., et al. 2019. Repeated anodal trans‐spinal direct current stimulation results in long‐term reduction of spasticity in mice with spinal cord injury. The Journal of physiology, Apr;597(8):2201-2223.
29028184 not listed Mahadevan, V., et al. 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. Elife, 6, p.e28270.
28683314 not listed Ruinetti-Vargas, G., et al. 2017. Periadolescent Maturation of GABAergic Hyperpolarization at the Axon Initial Segment. Cell Reports, 20(1), pp.21-29.
25733865 not listed Silayeva, L., et al. 2015 KCC2 activity is critical in limiting the onset and severity of status epilepticus. PNAS. Mar 2. pii: 201415126.
24478630 1:1000 Mòdol, L., et al. 2014. Differential effects on KCC2 expression and spasticity of ALS and traumatic injuries to motoneurons. Frontiers in cellular neuroscience, Jan 24; 8:7.
22171026 1:1000 Sarkar, J., et al. 2011. Neurosteroidogenesis is required for the physiological response to stress: role of neurosteroid-sensitive GABAA receptors. J Neurosci. 31(50):18198-210.


Western Blot: Rat
PMID Dilution Publication
29092909 1:1000 Conway, L.C., et al. 2017 N-ethylmaleimide increases KCC2 activity by modulating transporter phosphorylation. Journal of Biological Chemistry, Dec 29;292(52):21253-21263.
27639636 1:1000 Huang, Y.J., et al. 2016 Acute spinal cord injury (SCI) transforms how GABA affects nociceptive sensitization. Experimental Neurology, 285, pp.82-95.
26090759 1:1000 López-Álvarez, V. M., et al. 2015 Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury. Pain. Jun 18.
24861264 1:1000 Mòdol, L., et al. 2014 Neonatal allopregnanolone or finasteride administration modifies hippocampal K+ Cl− co-transporter expression during early development in male rats. The Journal of steroid biochemistry and molecular biology, Sep; 143:343-7.
24813295 1:1000 Mòdol, L., et al. 2014 Prevention of NKCC1 phosphorylation avoids downregulation of KCC2 in central sensory pathways and reduces neuropathic pain after peripheral nerve injury. PAIN, Aug; 155(8):1577-90.
21532577 not listed Lee, H.H., et al. 2014 NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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