Affinity purified from pooled serum. Learn more.

Potassium Channel, Kv2.2 Subunit Antibody

Our rabbit polyclonal Potassium Channel, Kv2.2 Subunit antibody is affinity purified from pooled serum. It is excellent for use in WB and IHC.

Catalog #: 1002-KV22 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Xenopus
Applications:
WB 1:1000 Gravagna et al 2008IHC 1:100-1:1000 Gravagna et al 2008 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
KCNB2
Molecular Weight:
~125 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1002-KV22, RRID:AB_2492211
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the subunit region of the voltage gated potassium channel, Kv2.2 of rat and Xenopus, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Synonyms: ExpandCollapse

• delayed rectifier potassium channel protein antibody
• KCNB2 antibody
• KCNB2_HUMAN antibody
• potassium channel Kv2.2 antibody
• potassium voltage gated channel subfamily B member 2 antibody
• Potassium voltage-gated channel subfamily B member 2 antibody
• Voltage-gated potassium channel subunit Kv2.2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat | Xenopus
Western Blot: Xenopus


Immunohistochemistry: Rat
PMID Dilution Publication
17379638 1:100-1:1000 Guan, D., et al. 2007 Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons. The Journal of Physiology, 581(3), pp.941-960.


Immunohistochemistry: Xenopus
PMID Dilution Publication
18680201 1:100 Gravagna, N.G., et al. 2008 Localization of Kv2. 2 protein in Xenopus laevis embryos and tadpoles. Journal of Comparative Neurology, 510(5), pp.508-524.


Western Blot: Xenopus
PMID Dilution Publication
18680201 1:100 Gravagna, N.G., et al. 2008 Localization of Kv2. 2 protein in Xenopus laevis embryos and tadpoles. Journal of Comparative Neurology, 510(5), pp.508-524.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…