Affinity purified from pooled serum. Learn more.

PLK1 (Thr210) Antibody

Catalog #: p202-210 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Bovine, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
PLK1
Molecular Weight:
~66 kDa
Cite This Antibody:
PhosphoSolutions Cat# p202-210, RRID:AB_2492209
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).

Synonyms: ExpandCollapse

• Cell cycle regulated protein kinase antibody
• PLK 1 antibody
• PLK antibody
• PLK-1 antibody
• plk1 antibody
• PLK1_HUMAN antibody
• Polo like kinase 1 antibody
• Polo-like kinase 1 antibody
• Serine/threonine protein kinase 13 antibody
• Serine/threonine protein kinase PLK1 antibody
• Serine/threonine-protein kinase 13 antibody
• Serine/threonine-protein kinase PLK1 antibody
• STPK 13 antibody
• STPK13 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25(5):2031-44. PMID: 15713655

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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PLK1 thr210 Antibody
Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK protein phosphorylated at Thr210 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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