Peripherin Antibody, Chicken

Catalog #: 1631-PER Categories: , , Datasheet:

$99.00$310.00

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 Great marker for classifying neurons!

Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:1000IHC 1:500-1:2000ICC 1:500-1:5000
Species:
Chicken
Gene Name:
PRPH
Molecular Weight:
~57 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1631-PER, RRID:AB_2492208
Antigen/Purification: ExpandCollapse

The antigen is a recombinant rat peripherin expressed in and purified from E. coli.

The antibody is total IgY fraction.

 

Biological Significance: ExpandCollapse

Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig’s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

Synonyms: ExpandCollapse

• NEF 4 antibody
• NEF4 antibody
• Neurofilament 4 (57kD) antibody
• Neurofilament 4 antibody
• Perf antibody
• PERI_HUMAN antibody
• Peripherin antibody
• PRPH 1 antibody
• prph antibody
• PRPH1 antibody

Storage

100 µl liquid. Contains 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat PC12 cells and fibroblasts
Newborn rat neurons and glia

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88. PMID: 11113316

Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. J. Cell Biol. 160(6):939-49. PMID: 12642616

Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6. PMID: 1729686

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Peripherin Antibody, Chicken
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.

Click here to view our Western blotting and lysate preparation protocols.

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