Parkin (Ser101) Antibody

Catalog #: p197-101 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Reactivity:
Human, Bovine, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
PARK2
Molecular Weight:
~52 kDa
Cite This Antibody:
PhosphoSolutions Cat# p197-101, RRID:AB_2492202
Antigen/Purification: ExpandCollapse

The antigen is a Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human parkin.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).

Synonyms: ExpandCollapse

• AR JP antibody
• E3 ubiquitin ligase antibody
• E3 ubiquitin protein ligase parkin antibody
• E3 ubiquitin-protein ligase parkin antibody
• FRA6E antibody
• LPRS 2 antibody
• LPRS2 antibody
• PARK 2 antibody
• Park2 antibody
• Parkin 2 antibody
• Parkinson disease (autosomal recessive juvenile) 2 antibody
• Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody
• Parkinson disease protein 2 antibody
• Parkinson juvenile disease protein 2 antibody
• Parkinson protein 2 E3 ubiquitin protein ligase antibody
• Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody
• PDJ antibody
• PRKN 2 antibody
• PRKN antibody
• PRKN2 antibody
• PRKN2_HUMAN antibody
• Ubiquitin E3 ligase PRKN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Rubio de la Torre E, Luzón-Toro B, Forte-Lago I, Minguez-Castellanos A, Ferrer I, Hilfiker S. (2009) Combined kinase inhibition modulates parkin inactivation. Hum Mol Genet. Mar 1;18(5):809-23. PMID: 19050041

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Parkin Ser101 Antibody
Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.

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