Park7 (DJ-1) Antibody

Catalog #: 1597-park7 Categories: , , , Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:5000ICC 1:1000-1:5000
Species:
Mouse Monoclonal
Gene Name:
PARK7
Molecular Weight:
~21 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1597-Park7, RRID:AB_2492201
Antigen/Purification: ExpandCollapse

The antigen is a full length recombinant human park7 expressed in and purified from E. coli.

The antibody is Protein G purified from tissue culture supernatant.

Biological Significance: ExpandCollapse

Park7, also known as DJ-1, is a member of the peptidase C56 family of proteins and is thought to function as a molecular chaperone. Mutations in park7 have been associated with autosomal recessive, early onset Parkinson’s disease (Bonifati et al., 2003). Recently, park7 has been shown to inhibit microtubule associated protein 1B aggregation thus leading to neuronal apoptosis (Wang et al., 2011).

Synonyms: ExpandCollapse

• CAP1 antibody
• DJ-1 antibody
• DJ1 antibody
• DJ1 protein antibody
• Epididymis secretory sperm binding protein Li 67p antibody
• FLJ27376 antibody
• FLJ34360 antibody
• FLJ92274 antibody
• HEL S 67p antibody
• Oncogene DJ1 antibody
• OTTHUMP00000001348 antibody
• OTTHUMP00000001349 antibody
• OTTHUMP00000001350 antibody
• OTTHUMP00000001351 antibody
• PARK7 antibody
• PARK7_HUMAN antibody
• Parkinson disease (autosomal recessive, early onset) 7 antibody
• Parkinson disease protein 7 antibody
• Parkinson protein 7 antibody
• Protein DJ-1 antibody
• SP22 antibody

Storage

100 µl in PBS + 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Bonifati V, Rizzu P, van Baren MJ, Schaap O, Breedveld GJ , Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M, van Dongen JW, Vanacore N, van Swieten JC, Brice A, Meco G, van Duijn CM, Oostra BA, Heutink P. (2003) Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism. Science. Jan 10;299(5604):256-9. PMID: 12446870

Wang Z, Zhang Y, Zhang S, Guo Q, Tan Y, Wang X, Xiong R, Ding J, Chen S. (2011) DJ-1 can inhibit microtubule associated protein 1 B formed aggregates. Mol Neurodegener. Jun 6;6:38. PMID: 21645326

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Park7 (DJ-1) Antibody
Western blot of rat kidney lysate showing specific immunolabeling of the ~21 kDa Park7 protein.

Click here to view our Western blotting and lysate preparation protocols.
Park7 (DJ-1) Antibody
Immunoflourescence of HeLa cells stained with Anti-Park7 (cat # 1597-PARK, green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin (cat #2105-VIM, red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.

Download the protocol used for this image:

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