Affinity purified from pooled serum. Learn more.

PanK4 (Ser63) Antibody

We are the original manufacturer of our rabbit polyclonal PanK4 (Ser63) phosphospecific antibody, affinity purified from pooled serum. It is optimized in WB.

Catalog #: p188-63 Categories: , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Expected Reactivity:
Hamster, Rat
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~86 kDa
Cite This Antibody:
PhosphoSolutions Cat# p188-63, RRID:AB_2636991
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser63 of mouse PanK4.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967).  There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001).  PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia.  PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001).  Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005).  There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.

Synonyms: ExpandCollapse

• DKFZp547M242 antibody
• FLJ10782 antibody
• hPanK 4 antibody
• hPanK4 antibody
• PANK 4 antibody
• Pantothenate kinase 4 antibody
• Pantothenic acid kinase antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Abiko, Y. Investigations on pantothenic acid and its related compounds. IX. Biochemical Studies. 4. Separation and substrate specificity of pantothenate kinase and phosphopantothenoylcysteine synthetase. J. Biochem (Tokyo) 61, 290-299 (1967). PMID: 6053309

Zhou B, Westaway S, Levinson B, Johnson M, Gitschier J, Hayflick S. A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome. Nat Genet 28, 345-349 (2001). PMID: 11479594

Li Y, Chang Y, Zhang L, Feng Q, Liu Z, Zhang Y, Zuo J, Meng Y, Fang F. High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2). Mol Cell Biochem 277, 117-125 (2005). PMID: 16132722

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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