Affinity purified from pooled serum. Learn more.

PanK4 (Ser63) Antibody

Catalog #: p188-63 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Hamster, Mouse, Rat
Applications:
WB 1:1,000
Species:
Rabbit Polyclonal
Molecular Reference:
~86 kDa
Cite This Antibody:
PhosphoSolutions Cat# p188-63, RRID:AB_2636991
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser63 of mouse PanK4.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967).  There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001).  PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia.  PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001).  Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005).  There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Abiko, Y. Investigations on pantothenic acid and its related compounds. IX. Biochemical Studies. 4. Separation and substrate specificity of pantothenate kinase and phosphopantothenoylcysteine synthetase. J. Biochem (Tokyo) 61, 290-299 (1967).

Zhou B, Westaway S, Levinson B, Johnson M, Gitschier J, Hayflick S. A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome.  Nat Genet 28, 345-349 (2001).

Li Y, Chang Y, Zhang L, Feng Q, Liu Z, Zhang Y, Zuo J, Meng Y, Fang F. High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2).  Mol Cell Biochem 277, 117-125 (2005).

PanK4 Ser63 Antibody
Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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