Affinity purified from pooled serum. Learn more.

PAK-1,2,3 (Thr402) Antibody

Catalog #: p187-402 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
PAK1-3
Molecular Weight:
~68 kDa to ~70 kDa
Cite This Antibody:
PhosphoSolutions Cat# p187-402, RRID:AB_2492200
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: ?-PAK (also known as PAK-1) and ?-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

Synonyms: ExpandCollapse

• Alpha PAK antibody
• Beta PAK antibody
• bPAK antibody
• CDKN1A antibody
• Gamma PAK antibody
• hPAK3 antibody
• MRX30 antibody
• MRX47 antibody
• Oligophrenin 3 antibody
• OPHN3 antibody
• P21 (CDKN1A) activated kinase 2 antibody
• P21 (CDKN1A) activated kinase 3 antibody
• p21 activated kinase 1 antibody
• p21 activated kinase 2 antibody
• p21 activated kinase 3 antibody
• P21 protein (Cdc42/Rac) activated kinase 1 antibody
• P21 protein (Cdc42/Rac) activated kinase 2 antibody
• P21 protein (Cdc42/Rac) activated kinase 3 antibody
• P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
• P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
• P58 antibody
• P65 PAK antibody
• PAK 2 antibody
• PAK 3 antibody
• PAK1 antibody
• PAK2 antibody
• PAK3 antibody
• PAK3beta antibody
• PAK65 antibody
• PAKalpha antibody
• PAKgamma antibody
• S6/H4 kinase antibody
• Serine/threonine protein kinase PAK 1 antibody
• Serine/threonine protein kinase PAK 2 antibody
• Serine/threonine protein kinase PAK 3 antibody
• Serine/threonine protein kinase PAK1 antibody
• Serine/threonine protein kinase PAK2 antibody
• Serine/threonine protein kinase PAK3 antibody
• STE20 homolog, yeast antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Nie, J., Lilley, B.N., Pan, Y.A., Faruque, O., Liu, X., Zhang, W., Sanes, J.R., Han, X. and Shi, Y., 2013. SAD-A potentiates glucose-stimulated insulin secretion as a mediator of glucagon-like peptide 1 response in pancreatic ? cells. Molecular and cellular biology, 33(13), pp.2527-2534.  PMID: 23629625

Jia Nie, Chao Sun, Omar Faruque, Guangming Ye, Jia Li, Qiangrong Liang, Zhijie Chang, Wannian Yang, Xiao Han, and Yuguang Shi (2012) Synapses of Amphids Defective (SAD-A) Kinase Promotes Glucose-stimulated Insulin Secretion through Activation of p21-activated Kinase (PAK1) in Pancreatic ?-Cells. J. Biol. Chem. 287:26435- 26444. PMID: 22669945

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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PAK-1,2,3 thr402
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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