Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~ 70 kDa
- Cite This Antibody:
- PhosphoSolutions Cat # p1705-398, RRID:AB_2629470
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr398 of Drosophila p70 S6K protein.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Biological Significance: ExpandCollapse
p70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009). p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009).