Affinity purified from pooled serum. Learn more.

p70 S6K (Thr398) Antibody

Catalog #: p1705-398 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Drosophila
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Molecular Reference:
~ 70 kDa
Cite This Antibody:
PhosphoSolutions Cat # p1705-398, RRID:AB_2629470
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr398 of Drosophila  p70 S6K protein.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Biological Significance: ExpandCollapse

p70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and  is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009). p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Tsokanos, F.F., Albert, M.A., Demetriades, C., Spirohn, K., Boutros, M. and Teleman, A.A., 2016. eIF4A inactivatesTORC1 in response to amino acid starvation. The EMBO journal, p.e201593118. PMID: 26988032

p70 s6k thr398
Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

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