Affinity purified from pooled serum. Learn more.

p62 (Thr269, Ser272) Antibody

We are the original manufacturer of our rabbit polyclonal p62 (Thr269, Ser272) phospho antibody, affinity purified from pooled serum. It is optimized in WB.

Catalog #: p196-269 Categories: , , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Expected Reactivity:
Non-human primate
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~62 kDa
Cite This Antibody:
PhosphoSolutions Cat# p196-269, RRID:AB_2492198
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 of human p62.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The protein scaffold and signaling regulator p62 (also known as sequestosome1 (SQSTM1)) is important in critical cellular functions, including bone homeostasis, obesity, and cancer, because of its interactions with various signaling intermediaries. p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at Thr269 and Ser272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Moscat et al., 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation (Moscat et al., 2011).

Synonyms: ExpandCollapse

• A170 antibody
• DMRV antibody
• EBI 3 associated protein of 60 kDa antibody
• EBI 3 associated protein p60 antibody
• EBI3 associated protein of 60 kDa antibody
• EBI3 associated protein p60 antibody
• EBI3-associated protein of 60 kDa antibody
• EBIAP antibody
• FTDALS3 antibody
• MGC127197 antibody
• ORCA antibody
• OSF-6 antibody
• Osi antibody
• OSIL antibody
• Oxidative stress induced like antibody
• p60 antibody
• p62 antibody
• p62B antibody
• Paget disease of bone 3 antibody
• PDB 3 antibody
• PDB3 antibody
• Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
• Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
• Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
• PKC-zeta-interacting protein antibody
• Protein kinase C-zeta-interacting protein antibody
• Sequestosome 1 antibody
• Sequestosome-1 antibody
• SQSTM 1 antibody
• SQSTM_HUMAN antibody
• Sqstm1 antibody
• STAP antibody
• STONE14 antibody
• Ubiquitin binding protein p62 antibody
• Ubiquitin-binding protein p62 antibody
• ZIP 3 antibody
• ZIP antibody
• ZIP3 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
29930081not listed Zhang, C., et al. 2018 p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14).

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Learn How Your Review May Be Used

You may also like…