Affinity purified from pooled serum. Learn more.

p62 (Thr269 Ser272) Antibody

Catalog #: p196-269 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Human, Non-human primate
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
SQSTM1
Molecular Reference:
~62 kDa
Cite This Antibody:
PhosphoSolutions Cat# p196-269, RRID:AB_2492198
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 of human p62.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The protein scaffold and signaling regulator p62 (also known as sequestosome1 (SQSTM1)) is important in critical cellular functions, including bone homeostasis, obesity, and cancer, because of its interactions with various signaling intermediaries. p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at Thr<sup>269</sup> and Ser<sup>272</sup>, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Moscat et al., 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation (Moscat et al., 2011).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Zhang, C., Gao, J., Li, M., Deng, Y. and Jiang, C., 2018. p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14). PMID: 29930081
p62 Thr269 Ser272 Antibody
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

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