Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- 100% Homology:
- Human, Non-human primate
- WB 1:1,000
- Gene Name:
- Molecular Reference:
- ~62 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p196-269, RRID:AB_2492198
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 of human p62.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
The protein scaffold and signaling regulator p62 (also known as sequestosome1 (SQSTM1)) is important in critical cellular functions, including bone homeostasis, obesity, and cancer, because of its interactions with various signaling intermediaries. p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at Thr<sup>269</sup> and Ser<sup>272</sup>, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Moscat et al., 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation (Moscat et al., 2011).