Affinity purified from pooled serum. Learn more.

p62 (Ser28) Antibody

Catalog #: p196-28 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Species Reactivity:
Non-human primate
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
SQSTM1
Molecular Weight:
~48 kDa
Cite This Antibody:
PhosphoSolutions Cat# p196-28, RRID:AB_2492197
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser28 of p62.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004). Phosphorylation of Ser28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease.

Synonyms: ExpandCollapse

• A170 antibody
• DMRV antibody
• EBI 3 associated protein of 60 kDa antibody
• EBI 3 associated protein p60 antibody
• EBI3 associated protein of 60 kDa antibody
• EBI3 associated protein p60 antibody
• EBI3-associated protein of 60 kDa antibody
• EBIAP antibody
• FTDALS3 antibody
• MGC127197 antibody
• ORCA antibody
• OSF-6 antibody
• Osi antibody
• OSIL antibody
• Oxidative stress induced like antibody
• p60 antibody
• p62 antibody
• p62B antibody
• Paget disease of bone 3 antibody
• PDB 3 antibody
• PDB3 antibody
• Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
• Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
• Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
• PKC-zeta-interacting protein antibody
• Protein kinase C-zeta-interacting protein antibody
• Sequestosome 1 antibody
• Sequestosome-1 antibody
• SQSTM 1 antibody
• SQSTM_HUMAN antibody
• Sqstm1 antibody
• STAP antibody
• STONE14 antibody
• Ubiquitin binding protein p62 antibody
• Ubiquitin-binding protein p62 antibody
• ZIP 3 antibody
• ZIP antibody
• ZIP3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Nogalska, A., Terracciano, C., D’Agostino, C., Engel, W.K. and Askanas, V., 2009. p62/SQSTM1 is overexpressed and prominently accumulated in inclusions of sporadic inclusion-body myositis muscle fibers, and can help differentiating it from polymyositis and dermatomyositis. Acta neuropathologica, 118(3), pp.407-413. PMID: 19557423

Nakaso, K., Yoshimoto, Y., Nakano, T., Takeshima, T., Fukuhara, Y., Yasui, K., Araga, S., Yanagawa, T., Ishii, T. and Nakashima, K., 2004. Transcriptional activation of p62/A170/ZIP during the formation of the aggregates: possible mechanisms and the role in Lewy body formation in Parkinson’s disease. Brain research, 1012(1-2), pp.42-51. PMID: 15158159

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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p62 ser28 antibody
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Click here to view our Western blotting and lysate preparation protocols.

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